PURPOSE: To establish an experimental model of traumatic ulcer in rat cheek mucosa for utilization in future alternative therapy studies. METHODS: A total of 60 adult male rats (250 - 300g) were used. Ulceration of the left cheek mucosa was provoked by abrasion using a nº 15 scalpel blade. The animals were observed for 10 days, during which they were weighed and their ulcers were measured. The histological characteristics were analyzed and scored according to the ulcer phase. In the statistical analysis, a value of p<0.01 was considered a statistically significant response in all cases. RESULTS: During the five first days, the animals lost weight (Student t test, p<0.01). The ulcerated area receded linearly over time and was almost completely cicatrized after 10 days (ANOVA, Tendency posttest, p<0.0001). Groups on days 1, 2 and 3 days displayed similar results, but a decrease in scores were observed after the 4th day. CONCLUSION: The proposed cheek mucosa ulcer model in rats can be considered an efficient, low-cost, reliable, and reproducible method.
PURPOSE: To establish an experimental model of traumatic ulcer in rat cheek mucosa for utilization in future alternative therapy studies. METHODS: A total of 60 adult male rats (250 - 300g) were used. Ulceration of the left cheek mucosa was provoked by abrasion using a nº 15 scalpel blade. The animals were observed for 10 days, during which they were weighed and their ulcers were measured. The histological characteristics were analyzed and scored according to the ulcer phase. In the statistical analysis, a value of p<0.01 was considered a statistically significant response in all cases. RESULTS: During the five first days, the animals lost weight (Student t test, p<0.01). The ulcerated area receded linearly over time and was almost completely cicatrized after 10 days (ANOVA, Tendency posttest, p<0.0001). Groups on days 1, 2 and 3 days displayed similar results, but a decrease in scores were observed after the 4th day. CONCLUSION: The proposed cheek mucosa ulcer model in rats can be considered an efficient, low-cost, reliable, and reproducible method.
Authors: Paulo Goberlânio de Barros Silva; Érian Bezerra Belo de Codes; Milena Oliveira Freitas; Joyce Ohana de Lima Martins; Ana Paula Negreiros Nunes Alves; Fabrício Bitu Sousa Journal: J Oral Maxillofac Pathol Date: 2018 Sep-Dec
Authors: Dayrine Silveira de Paula; Lia Vila Real Lima; Roberta Barroso Cavalcante; Paulo Goberlânio de Barros Silva; Ana Paula Negreiros Nunes Alves; Bruno Rocha da Silva Journal: Saudi Dent J Date: 2021-05-08
Authors: T F G Souza; T M Pierdoná; F S Macedo; P E A Aquino; G F P Rangel; R S Duarte; L M A Silva; G S B Viana; A P N N Alves; R C Montenegro; D V Wilke; E R Silveira; N M N Alencar Journal: Braz J Med Biol Res Date: 2021-05-31 Impact factor: 2.590