Samah M Abdel-Hamid1, Hend N Al-Lithy. 1. Department of Clinical and Chemical Pathology, Faculty of Medicine, Kasr El-Aini hospital, Cairo University, Cairo, Egypt. samah_cpath@yahoo.com
Abstract
INTRODUCTION: B cell activating factor (BAFF) is a ligand belonging to the tumor necrosis factor family and has an important role in B cells development, survival, and immunoglobulin-production. This study was aimed to study the association between haplotypes in the 5' regulatory region of the BAFF gene and susceptibility to idiopathic thrombocytopenic purpura (ITP). METHODS: BAFF gene polymorphisms expression at positions (-871C>T, -2701 T>A and -2841T>C) of 5' regulatory region were determined with polymerase chain reaction restriction fragment length polymorphism technique in 40 chronic (ITP) patients and in 50 age- and sex-matched normal volunteers as a control group. RESULTS: The difference between chronic ITP patients and control subjects as regards -871C>T polymorphism expression was highly statistically significant with higher expression of T allele in ITP patients than control group (P < 0.001). However, the other 2 polymorphisms did not show significant difference between the 2 groups. -871C>T polymorphism was associated with increased risk of chronic ITP with an odds ratio 18.857; however, the other 2 polymorphisms were not associated with an equivalent effect. CONCLUSION: -871C>T BAFF gene polymorphism expression had a significant association with chronic ITP disease.
INTRODUCTION:B cell activating factor (BAFF) is a ligand belonging to the tumor necrosis factor family and has an important role in B cells development, survival, and immunoglobulin-production. This study was aimed to study the association between haplotypes in the 5' regulatory region of the BAFF gene and susceptibility to idiopathic thrombocytopenic purpura (ITP). METHODS:BAFF gene polymorphisms expression at positions (-871C>T, -2701 T>A and -2841T>C) of 5' regulatory region were determined with polymerase chain reaction restriction fragment length polymorphism technique in 40 chronic (ITP) patients and in 50 age- and sex-matched normal volunteers as a control group. RESULTS: The difference between chronic ITP patients and control subjects as regards -871C>T polymorphism expression was highly statistically significant with higher expression of T allele in ITP patients than control group (P < 0.001). However, the other 2 polymorphisms did not show significant difference between the 2 groups. -871C>T polymorphism was associated with increased risk of chronic ITP with an odds ratio 18.857; however, the other 2 polymorphisms were not associated with an equivalent effect. CONCLUSION: -871C>T BAFF gene polymorphism expression had a significant association with chronic ITP disease.
Authors: M Marín-Rosales; A Cruz; D C Salazar-Camarena; E Santillán-López; N Espinoza-García; J F Muñoz-Valle; M G Ramírez-Dueñas; E Oregón-Romero; G Orozco-Barocio; C A Palafox-Sánchez Journal: Clin Exp Med Date: 2019-02-11 Impact factor: 5.057