| Literature DB >> 21536074 |
Camila Zanluca1, Luan Renato Dos Passos Aires, Paula Pazzini Mueller, Vanessa Valgas Dos Santos, Maria Luiza Carrieri, Aguinaldo Roberto Pinto, Carlos Roberto Zanetti.
Abstract
Ten monoclonal antibodies (MAbs) against rabies virus, including IgG3κ, IgG2aκ, IgMκ, and an IgG2bκ isotype, were produced and characterized using neutralization, ELISA, immunodot-blot, and immunofluorescence assays. MAb 8D11, which recognized rabies virus glycoprotein, was found to neutralize rabies virus in vitro. When submitted to an immunofluorescence assay, seven MAbs showed different reactivity against 35 Brazilian rabies virus isolates. Three MAbs (LIA 02, 3E6, and 9C7) only failed to recognize one or two virus isolates, whereas MAb 6H8 was found to be reactive against all virus isolates tested. MAbs were also evaluated for their immunoreactivity against fixed rabies virus strains present in human and veterinary commercial vaccines. MAbs LIA 02, 6H8, and 9C7 reacted against all vaccine strains, while the remaining MAbs recognized at least 76% of vaccine strains tested. This research provides a set of MAbs with potential application for improving existing or developing new diagnostic tests and immunoassays.Entities:
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Year: 2011 PMID： 21536074 DOI： 10.1016/j.jviromet.2011.04.019
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014