| Literature DB >> 2153053 |
M K Bhattacharyya1, A M Smith, T H Ellis, C Hedley, C Martin.
Abstract
We describe the cloning of the r (rugosus) locus of pea (Pisum sativum L.), which determines whether the seed is round or wrinkled. Wrinkled (rr) seeds lack one isoform of starch-branching enzyme (SBEI), present in round (RR or Rr) seeds. A major polymorphism in the SBEI gene between near-isogenic RR and rr lines shows 100% cosegregation with the r locus, establishing that the SBEI gene is at the r locus. An aberrant transcript for SBEI is produced in rr embryos. In rr lines the SBEI gene is interrupted by a 0.8 kb insertion that is very similar to the Ac/Ds family of transposable elements from maize. Failure to produce SBEI has complex metabolic consequences on starch, lipid, and protein biosynthesis in the seed.Entities:
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Year: 1990 PMID： 2153053 DOI： 10.1016/0092-8674(90)90721-p
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582