| Literature DB >> 21528460 |
Yohei Kirino1, Anastassios Vourekas, Eugene Khandros, Zissimos Mourelatos.
Abstract
Piwi interacting RNAs (piRNAs) are small (∼25 to ∼30 nucleotide) and are expressed in the germline. piRNAs bind to the Piwi subclade of Argonaute proteins and form the core ribonucleoproteins (RNPs) of piRNPs. We describe a method for the massive identification of piRNAs from immunopurified piRNPs. This strategy may also be used for immunopurification and directional sequencing of RNAs from other RNPs that contain small RNAs.Entities:
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Year: 2011 PMID： 21528460 DOI： 10.1007/978-1-61779-046-1_18
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745