OBJECTIVE: Th1 activation and regulatory T (Treg) cell suppression have been observed in acute coronary syndrome (ACS), including unstable angina (UA) and acute myocardial infarction (AMI). However, the role of Th17 cell or IL-17A remains controversial in ACS patients, and little is known about the role of recently discovered Th17/Th1 cells, a subset of Th17 cells, in coronary artery disease (CAD). The purpose of this study is to investigate functional changes of Th17/Th1, Th17, Th1, Th2 and Treg cells in ACS patients. METHODS: The contents of Th17/Th1, Th17, Th1, Th2 and Treg cells, related gene expression, and plasma cytokines from CAD and control patients with normal coronary arteries (NCA) were measured by flow cytometry, real-time quantitative PCR and ELISA. RESULTS: Th17/Th1 and Th1 cell contents and related gene expression (T-bet, IFN-γ, STAT4, RORγt, STAT3 and IL-17) were significantly increased in ACS patients, whereas plasma IFN-γ only increased in CAD patients. In contrast, Treg cell population, Foxp3 levels, and plasma TGF-β1 were decreased in ACS patients compared with stable angina (SA) and NCA patients. CONCLUSION: The study showed activation of Th17/Th1 and Th1 cell in ACS patients, which may provide insight into the mechanisms underlying culprit plaque relevant T-cell activation in ACS patients.
OBJECTIVE:Th1 activation and regulatory T (Treg) cell suppression have been observed in acute coronary syndrome (ACS), including unstable angina (UA) and acute myocardial infarction (AMI). However, the role of Th17 cell or IL-17A remains controversial in ACS patients, and little is known about the role of recently discovered Th17/Th1 cells, a subset of Th17 cells, in coronary artery disease (CAD). The purpose of this study is to investigate functional changes of Th17/Th1, Th17, Th1, Th2 and Treg cells in ACS patients. METHODS: The contents of Th17/Th1, Th17, Th1, Th2 and Treg cells, related gene expression, and plasma cytokines from CAD and control patients with normal coronary arteries (NCA) were measured by flow cytometry, real-time quantitative PCR and ELISA. RESULTS: Th17/Th1 and Th1 cell contents and related gene expression (T-bet, IFN-γ, STAT4, RORγt, STAT3 and IL-17) were significantly increased in ACS patients, whereas plasma IFN-γ only increased in CAD patients. In contrast, Treg cell population, Foxp3 levels, and plasma TGF-β1 were decreased in ACS patients compared with stable angina (SA) and NCA patients. CONCLUSION: The study showed activation of Th17/Th1 and Th1 cell in ACS patients, which may provide insight into the mechanisms underlying culprit plaque relevant T-cell activation in ACS patients.
Authors: Joabe S Pereira; Bárbara V Monteiro; Cassiano F Nonaka; Éricka J Silveira; Márcia C Miguel Journal: Int J Exp Pathol Date: 2012-08 Impact factor: 1.925
Authors: Jun Yang; Xiaoyang Yuan; Caixia Lv; Rong Bai; Le Zhang; Lei Ruang; Cuntai Zhang; Xiao-Qing Quan Journal: Am J Transl Res Date: 2016-12-15 Impact factor: 4.060
Authors: Ignacio M Seropian; Juan P Cerliani; Stefano Toldo; Benjamín W Van Tassell; Juan M Ilarregui; Germán E González; Mirian Matoso; Fadi N Salloum; Ryan Melchior; Ricardo J Gelpi; Juan C Stupirski; Alejandro Benatar; Karina A Gómez; Celina Morales; Antonio Abbate; Gabriel A Rabinovich Journal: Am J Pathol Date: 2012-11-09 Impact factor: 4.307