Literature DB >> 2152183

One-step fractionation method for isolating H1 histones from chromatin under nondenaturing conditions.

M Garcia-Ramirez1, S H Leuba, J Ausio.   

Abstract

By combining conventional methods of chromatin preparation with ion-exchange chromatography in CM-Sephadex C-25, we have been able to isolate and fractionate histone H1 variants from different sources. This method of fractionation is very simple and allows one to obtain, very rapidly, large amounts of these histones in a native nondenatured conformation.

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Year:  1990        PMID： 2152183     DOI： 10.1016/1046-5928(90)90043-x

Source DB:  PubMed          Journal:  Protein Expr Purif        ISSN： 1046-5928            Impact factor:   1.650


  9 in total

1.  The preferential binding of histone H1 to DNA scaffold-associated regions is determined by its C-terminal domain.

Authors:  Alicia Roque; Mary Orrego; Imma Ponte; Pedro Suau
Journal:  Nucleic Acids Res       Date:  2004-11-23       Impact factor: 16.971

2.  Linker histone tails and N-tails of histone H3 are redundant: scanning force microscopy studies of reconstituted fibers.

Authors:  S H Leuba; C Bustamante; K van Holde; J Zlatanova
Journal:  Biophys J       Date:  1998-06       Impact factor: 4.033

3.  Binding of histones H1 and H5 and their globular domains to four-way junction DNA.

Authors:  P Varga-Weisz; J Zlatanova; S H Leuba; G P Schroth; K van Holde
Journal:  Proc Natl Acad Sci U S A       Date:  1994-04-26       Impact factor: 11.205

4.  Direct activation and anti-repression functions of GAL4-VP16 use distinct molecular mechanisms.

Authors:  J G Lyons; P Chambon
Journal:  Biochem J       Date:  1995-12-15       Impact factor: 3.857

5.  Histones H1 and H5 interact preferentially with crossovers of double-helical DNA.

Authors:  D Krylov; S Leuba; K van Holde; J Zlatanova
Journal:  Proc Natl Acad Sci U S A       Date:  1993-06-01       Impact factor: 11.205

6.  Coil-to-helix transitions in intrinsically disordered methyl CpG binding protein 2 and its isolated domains.

Authors:  Kristopher C Hite; Anna A Kalashnikova; Jeffrey C Hansen
Journal:  Protein Sci       Date:  2012-03-09       Impact factor: 6.725

7.  Interaction of chromatin with a histone H1 containing swapped N- and C-terminal domains.

Authors:  Jordana B Hutchinson; Manjinder S Cheema; Jason Wang; Krystal Missiaen; Ron Finn; Rodrigo Gonzalez Romero; John P H Th'ng; Michael Hendzel; Juan Ausió
Journal:  Biosci Rep       Date:  2015-04-27       Impact factor: 3.840

8.  A Quantitative Characterization of Nucleoplasmin/Histone Complexes Reveals Chaperone Versatility.

Authors:  Noelia Fernández-Rivero; Aitor Franco; Adrian Velázquez-Campoy; Edurne Alonso; Arturo Muga; Adelina Prado
Journal:  Sci Rep       Date:  2016-08-25       Impact factor: 4.379

9.  Differential affinity of mammalian histone H1 somatic subtypes for DNA and chromatin.

Authors:  Mary Orrego; Imma Ponte; Alicia Roque; Natascha Buschati; Xavier Mora; Pedro Suau
Journal:  BMC Biol       Date:  2007-05-11       Impact factor: 7.431
  9 in total

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