| Literature DB >> 21521610 |
Carolyn W-H Wu1, Olga Vasalatiy, Ning Liu, Haitao Wu, Sarah Cheal, Der-Yow Chen, Alan P Koretsky, Gary L Griffiths, Roger B H Tootell, Leslie G Ungerleider.
Abstract
Traditional studies of neuroanatomical connections require injection of tracer compounds into living brains, then histology of the postmortem tissue. Here, we describe and validate a compound that reveals neuronal connections in vivo, using MRI. The classic anatomical tracer CTB (cholera-toxin subunit-B) was conjugated with a gadolinium-chelate to form GdDOTA-CTB. GdDOTA-CTB was injected into the primary somatosensory cortex (S1) or the olfactory pathway of rats. High-resolution MR images were collected at a range of time points at 11.7T and 7T. The transported GdDOTA-CTB was visible for at least 1 month post-injection, clearing within 2 months. Control injections of non-conjugated GdDOTA into S1 were not transported and cleared within 1-2 days. Control injections of Gd-Albumin were not transported either, clearing within 7 days. These MR results were verified by classic immunohistochemical staining for CTB, in the same animals. The GdDOTA-CTB neuronal transport was target specific, monosynaptic, stable for several weeks, and reproducible.Entities:
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Year: 2011 PMID： 21521610 PMCID： PMC3419536 DOI： 10.1016/j.neuron.2011.03.010
Source DB: PubMed Journal: Neuron ISSN： 0896-6273 Impact factor: 17.173