OBJECTIVE: Fibroblast growth factor (FGF21) is a potent regulator of glucose and lipid metabolism. In rodents, the hepatic expression of FGF21 is controlled by fasting and a circadian regulation, but the physiological role and regulation of FGF21 in humans is not well established. Therefore, the objective of this study was to elucidate the 24-h profiling of plasma FGF21 during a 72-h fast. STUDY DESIGN: After an initial 12-h overnight fast, plasma levels of FGF21 together with circulating levels of glucose, insulin, glucagon and free fatty acids (FFA) were measured every 6 h during a 72-h fast in healthy female volunteers (n = 14). RESULTS: During the fast, plasma glucose and serum insulin gradually decreased whilst glucagon and FFA levels increased. Mean 24-h plasma FGF21 levels did not increase during the 72-h fast, but, despite large inter-individually variations, a significant circadian rhythm was observed. Thus, during the first day, plasma FGF21 increased from 125 pg/ml (16-142) at 8.30 a.m to peak levels of 224 pg/ml (88-326) at 2.30 a.m (P = 0·0215), the second day from 60 pg/ml (7-74) at 8.30 a.m to 268 pg/ml (103-410) at 2.30 a.m. (P = 0·0010) and the third day from 94 pg/ml (9-146) to 151 pg/ml (87-207) at 2.30 a.m. (P = 0·0049). Cortisol also showed circadian regulation and peaked at 8.30 a.m every day, 6 h after the observed FGF21 peak. CONCLUSION: Plasma FGF21 follows a circadian rhythm during a 72-h fast in healthy female subjects. The circadian regulation has a stronger impact on plasma FGF21 than the fasting status over 72-h period.
OBJECTIVE: Fibroblast growth factor (FGF21) is a potent regulator of glucose and lipid metabolism. In rodents, the hepatic expression of FGF21 is controlled by fasting and a circadian regulation, but the physiological role and regulation of FGF21 in humans is not well established. Therefore, the objective of this study was to elucidate the 24-h profiling of plasma FGF21 during a 72-h fast. STUDY DESIGN: After an initial 12-h overnight fast, plasma levels of FGF21 together with circulating levels of glucose, insulin, glucagon and free fatty acids (FFA) were measured every 6 h during a 72-h fast in healthy female volunteers (n = 14). RESULTS: During the fast, plasma glucose and serum insulin gradually decreased whilst glucagon and FFA levels increased. Mean 24-h plasma FGF21 levels did not increase during the 72-h fast, but, despite large inter-individually variations, a significant circadian rhythm was observed. Thus, during the first day, plasma FGF21 increased from 125 pg/ml (16-142) at 8.30 a.m to peak levels of 224 pg/ml (88-326) at 2.30 a.m (P = 0·0215), the second day from 60 pg/ml (7-74) at 8.30 a.m to 268 pg/ml (103-410) at 2.30 a.m. (P = 0·0010) and the third day from 94 pg/ml (9-146) to 151 pg/ml (87-207) at 2.30 a.m. (P = 0·0049). Cortisol also showed circadian regulation and peaked at 8.30 a.m every day, 6 h after the observed FGF21 peak. CONCLUSION: Plasma FGF21 follows a circadian rhythm during a 72-h fast in healthy female subjects. The circadian regulation has a stronger impact on plasma FGF21 than the fasting status over 72-h period.
Authors: Hassan S Dashti; Jordi Merino; Jacqueline M Lane; Yanwei Song; Caren E Smith; Toshiko Tanaka; Nicola M McKeown; Chandler Tucker; Dianjianyi Sun; Traci M Bartz; Ruifang Li-Gao; Hoirun Nisa; Sirimon Reutrakul; Rozenn N Lemaitre; Tahani M Alshehri; Renée de Mutsert; Lydia Bazzano; Lu Qi; Kristen L Knutson; Bruce M Psaty; Dennis O Mook-Kanamori; Vesna Boraska Perica; Marian L Neuhouser; Frank A J L Scheer; Martin K Rutter; Marta Garaulet; Richa Saxena Journal: Am J Clin Nutr Date: 2019-08-01 Impact factor: 7.045
Authors: Richard D Semba; Kai Sun; Josephine M Egan; Candace Crasto; Olga D Carlson; Luigi Ferrucci Journal: J Clin Endocrinol Metab Date: 2012-02-16 Impact factor: 5.958
Authors: Rucha Patel; Angie L Bookout; Lilia Magomedova; Bryn M Owen; Giulia P Consiglio; Makoto Shimizu; Yuan Zhang; David J Mangelsdorf; Steven A Kliewer; Carolyn L Cummins Journal: Mol Endocrinol Date: 2014-12-11
Authors: Pouneh K Fazeli; Mingyue Lun; Soo M Kim; Miriam A Bredella; Spenser Wright; Yang Zhang; Hang Lee; Ciprian Catana; Anne Klibanski; Parth Patwari; Matthew L Steinhauser Journal: J Clin Invest Date: 2015-11-03 Impact factor: 14.808
Authors: A Esteghamati; A Khandan; A Momeni; A Behdadnia; A Ghajar; M S Nikdad; S Noshad; M Nakhjavani; M Afarideh Journal: Ir J Med Sci Date: 2017-02-08 Impact factor: 1.568