Literature DB >> 21519369

A new method for sex determination based on detection of SRY, STS and amelogenin gene regions with simultaneous amplification of their homologous sequences by a multiplex PCR.

Toshio Morikawa1, Yuji Yamamoto, Satoru Miyaishi.   

Abstract

We have developed a new method for sex determination based on simultaneous detection of the SRY (sex-determining region Y), STS (steroid sulfatase) and amelogenin (AMELX and AMELY) gene regions and their homologous sequences. The sex of 246 blood samples was correctly determined by this method. An AMELY-deleted male sample, which would have been erroneously considered female based solely on analysis of the amelogenin locus, was successfully identified as male by the present method. The detection limit of this method was 63 pg of genomic DNA, and the male DNA component could be detected from mixed samples having a male:female ratio as low as 1:10. This method was useful for degraded DNA and possessed the human specificity. Practical application to 35 autopsy cases is described.

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Year:  2011        PMID： 21519369     DOI： 10.18926/AMO/45270

Source DB:  PubMed          Journal:  Acta Med Okayama        ISSN： 0386-300X            Impact factor:   0.892


  9 in total

1.  A novel method for sex determination by detecting the number of X chromosomes.

Authors:  Hiroaki Nakanishi; Hideki Shojo; Takeshi Ohmori; Masaaki Hara; Aya Takada; Noboru Adachi; Kazuyuki Saito
Journal:  Int J Legal Med       Date:  2014-08-27       Impact factor: 2.686

Review 2.  Alternatives to amelogenin markers for sex determination in humans and their forensic relevance.

Authors:  Hirak R Dash; Neha Rawat; Surajit Das
Journal:  Mol Biol Rep       Date:  2020-01-25       Impact factor: 2.316

3.  A qPCR-duplex assay for sex determination in ancient DNA.

Authors:  Anna Poma; Patrizia Cesare; Antonella Bonfigli; Anna Rita Volpe; Sabrina Colafarina; Giulia Vecchiotti; Alfonso Forgione; Osvaldo Zarivi
Journal:  PLoS One       Date:  2022-06-10       Impact factor: 3.752

4.  Sex Chromosomes Are Severely Disrupted in Gastric Cancer Cell Lines.

Authors:  Sooeun Oh; Kyoungmi Min; Myungshin Kim; Suk Kyeong Lee
Journal:  Int J Mol Sci       Date:  2020-06-28       Impact factor: 5.923

5.  Differential fate between oxytocin and vasopressin cells in the developing mouse brain.

Authors:  Amelie Soumier; Marie Habart; Guillaume Lio; Caroline Demily; Angela Sirigu
Journal:  iScience       Date:  2021-12-18

6.  Examining Sex Differences in the Human Placental Transcriptome During the First Fetal Androgen Peak.

Authors:  Amy E Braun; Kristin L Muench; Beatriz G Robinson; Angela Wang; Theo D Palmer; Virginia D Winn
Journal:  Reprod Sci       Date:  2020-11-04       Impact factor: 3.060

7.  Comparison of sexual dimorphism of permanent mandibular canine with mandibular first molar by odontometrics.

Authors:  Aditi Agrawal; Bhari Shranesha Manjunatha; Bhavik Dholia; Yousef Althomali
Journal:  J Forensic Dent Sci       Date:  2015 Sep-Dec

8.  Genetic sex determination assays in 53 mammalian species: Literature analysis and guidelines for reporting standardization.

Authors:  Karin Hrovatin; Tanja Kunej
Journal:  Ecol Evol       Date:  2017-12-13       Impact factor: 2.912

9.  Molecular characterization of mitochondrial Amerindian haplogroups and the amelogenin gene in human ancient DNA from three archaeological sites in Lambayeque - Peru.

Authors:  Jorge Victor Wilfredo Cachay Wester; Vanny Judith Soplapuco Vilchez; Carlos Eduardo Wester La Torre; Luis Alberto Rodriguez-Delfin
Journal:  Genet Mol Biol       Date:  2020-11-25       Impact factor: 1.771
  9 in total

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