OBJECTIVES: H-cadherin, functions as a tumor suppressor, is frequently silenced by promoter methylation in human cancers. The aim of this study was to evaluate the feasibility of using H-cadherin methylation in tumor tissues as a potential biomarker in patients with bladder transitional cell carcinoma (TCC). MATERIALS AND METHODS: We examined the methylation status of H-cadherin in 133 primary bladder TCC samples and 43 normal bladder epithelial tissues using methylation-specific polymerase chain reaction (MSP) and then analyzed the associations between H-cadherin methylation and clinicopathologic features as well as patients' outcome. RESULTS: H-cadherin methylation was detected in 47 (35.3%) bladder TCC samples, but not found in controls (P = 0.0000). Moreover, H-cadherin methylation was significantly associated with advanced stage (P = 0.0006), high grade (P = 0.0165), larger tumor size (P = 0.0225), tumor recurrence (P = 0.0106), and poor prognosis (P = 0.0000). In addition, multivariate analysis indicated that H-cadherin methylation is independently associated with poor outcome and had a relative risk of death of 3.832 (P = 0.0071, 95% confidence interval: 1.443-10.176). CONCLUSIONS: The results suggest that H-cadherin methylation may be used as a potential biomarker for the malignancy of bladder TCC and as an independent prognostic biomarker in patients with bladder TCC.
OBJECTIVES: H-cadherin, functions as a tumor suppressor, is frequently silenced by promoter methylation in humancancers. The aim of this study was to evaluate the feasibility of using H-cadherin methylation in tumor tissues as a potential biomarker in patients with bladder transitional cell carcinoma (TCC). MATERIALS AND METHODS: We examined the methylation status of H-cadherin in 133 primary bladder TCC samples and 43 normal bladder epithelial tissues using methylation-specific polymerase chain reaction (MSP) and then analyzed the associations between H-cadherin methylation and clinicopathologic features as well as patients' outcome. RESULTS: H-cadherin methylation was detected in 47 (35.3%) bladder TCC samples, but not found in controls (P = 0.0000). Moreover, H-cadherin methylation was significantly associated with advanced stage (P = 0.0006), high grade (P = 0.0165), larger tumor size (P = 0.0225), tumor recurrence (P = 0.0106), and poor prognosis (P = 0.0000). In addition, multivariate analysis indicated that H-cadherin methylation is independently associated with poor outcome and had a relative risk of death of 3.832 (P = 0.0071, 95% confidence interval: 1.443-10.176). CONCLUSIONS: The results suggest that H-cadherin methylation may be used as a potential biomarker for the malignancy of bladder TCC and as an independent prognostic biomarker in patients with bladder TCC.
Authors: R Maruyama; S Toyooka; K O Toyooka; K Harada; A K Virmani; S Zöchbauer-Müller; A J Farinas; F Vakar-Lopez; J D Minna; A Sagalowsky; B Czerniak; A F Gazdar Journal: Cancer Res Date: 2001-12-15 Impact factor: 12.701
Authors: Malcolm V Brock; Craig M Hooker; Emi Ota-Machida; Yu Han; Mingzhou Guo; Stephen Ames; Sabine Glöckner; Steven Piantadosi; Edward Gabrielson; Genevieve Pridham; Kristen Pelosky; Steven A Belinsky; Stephen C Yang; Stephen B Baylin; James G Herman Journal: N Engl J Med Date: 2008-03-13 Impact factor: 91.245
Authors: Andreas Widschwendter; Lennart Ivarsson; Anya Blassnig; Hannes M Müller; Heidi Fiegl; Annemarie Wiedemair; Elisabeth Müller-Holzner; Georg Goebel; Christian Marth; Martin Widschwendter Journal: Int J Cancer Date: 2004-03-20 Impact factor: 7.396
Authors: Morgan Rouprêt; Vincent Hupertan; David R Yates; Eva Comperat; James W F Catto; Mark Meuth; Amine Lackmichi; Sylvie Ricci; Roger Lacave; Bernard Gattegno; François Richard; Freddie C Hamdy; Olivier Cussenot Journal: BJU Int Date: 2008-03-04 Impact factor: 5.588
Authors: Ekaterina Olkhov-Mitsel; Andrea J Savio; Ken J Kron; Vaijayanti V Pethe; Thomas Hermanns; Neil E Fleshner; Bas W van Rhijn; Theodorus H van der Kwast; Alexandre R Zlotta; Bharati Bapat Journal: Transl Oncol Date: 2017-02-03 Impact factor: 4.243