PURPOSE: To determine the ability of the principal diffusion tensor imaging (DTI) indices to predict the underlying histopathology evaluated with immunofluorescent assay (IFA). MATERIALS AND METHODS: Conventional T2 and 3D multishot-diffusion weighted echoplanar imaging (3D ms-DWEPI) was performed on a fixed, ex vivo human cervical spinal cord (CSC) from a patient with a history of multiple sclerosis (MS). In all, 170 regions of interest (ROIs) were selected within the white matter and categorized as a high intensity lesion (HIL), low intensity lesion (LIL), and normal-appearing white matter (NAWM). The longitudinal diffusivity (λl), radial diffusivity (λr), and fractional anisotropy (FA) were obtained from each ROI. The underlying histopathology was then evaluated using immunofluorescent assay with antibodies directed to myelin and neurofilament staining. RESULTS: The mean values for λl and λr were significantly elevated within HIL relative to NAWM and LIL. IFA analysis of HIL demonstrated significant demyelination, without significant if any axon loss. The FA values were significantly reduced in HIL and LILs. FA values were also reduced in lesions with increased λl and λr values relative to normal. CONCLUSION: Aberrant λl, λr, and FA relative to normal values are strong indicators of demyelination. DTI indices are not specific for axon loss. IFA analysis is a reliable method to demonstrate myelin and axon pathology within the ex vivo setting.
PURPOSE: To determine the ability of the principal diffusion tensor imaging (DTI) indices to predict the underlying histopathology evaluated with immunofluorescent assay (IFA). MATERIALS AND METHODS: Conventional T2 and 3D multishot-diffusion weighted echoplanar imaging (3D ms-DWEPI) was performed on a fixed, ex vivo human cervical spinal cord (CSC) from a patient with a history of multiple sclerosis (MS). In all, 170 regions of interest (ROIs) were selected within the white matter and categorized as a high intensity lesion (HIL), low intensity lesion (LIL), and normal-appearing white matter (NAWM). The longitudinal diffusivity (λl), radial diffusivity (λr), and fractional anisotropy (FA) were obtained from each ROI. The underlying histopathology was then evaluated using immunofluorescent assay with antibodies directed to myelin and neurofilament staining. RESULTS: The mean values for λl and λr were significantly elevated within HIL relative to NAWM and LIL. IFA analysis of HIL demonstrated significant demyelination, without significant if any axon loss. The FA values were significantly reduced in HIL and LILs. FA values were also reduced in lesions with increased λl and λr values relative to normal. CONCLUSION: Aberrant λl, λr, and FA relative to normal values are strong indicators of demyelination. DTI indices are not specific for axon loss. IFA analysis is a reliable method to demonstrate myelin and axon pathology within the ex vivo setting.
Authors: G J Nijeholt; E Bergers; W Kamphorst; J Bot; K Nicolay; J A Castelijns; J H van Waesberghe; R Ravid; C H Polman; F Barkhof Journal: Brain Date: 2001-01 Impact factor: 13.501
Authors: M Filippi; M Bozzali; M Rovaris; O Gonen; C Kesavadas; A Ghezzi; V Martinelli; R I Grossman; G Scotti; G Comi; A Falini Journal: Brain Date: 2003-02 Impact factor: 13.501
Authors: D J Werring; D Brassat; A G Droogan; C A Clark; M R Symms; G J Barker; D G MacManus; A J Thompson; D H Miller Journal: Brain Date: 2000-08 Impact factor: 13.501
Authors: J P Mottershead; K Schmierer; M Clemence; J S Thornton; F Scaravilli; G J Barker; P S Tofts; J Newcombe; M L Cuzner; R J Ordidge; W I McDonald; D H Miller Journal: J Neurol Date: 2003-11 Impact factor: 4.849
Authors: Carmen Tur; Olivia Goodkin; Daniel R Altmann; Thomas M Jenkins; Katherine Miszkiel; Alessia Mirigliani; Camilla Fini; Claudia A M Gandini Wheeler-Kingshott; Alan J Thompson; Olga Ciccarelli; Ahmed T Toosy Journal: Brain Date: 2016-02-17 Impact factor: 13.501
Authors: Jiwon Oh; Kathleen Zackowski; Min Chen; Scott Newsome; Shiv Saidha; Seth A Smith; Marie Diener-West; Jerry Prince; Craig K Jones; Peter C M Van Zijl; Peter A Calabresi; Daniel S Reich Journal: Mult Scler Date: 2012-08-13 Impact factor: 6.312
Authors: A Blaschek; D Keeser; S Müller; I K Koerte; A Sebastian Schröder; W Müller-Felber; F Heinen; B Ertl-Wagner Journal: AJNR Am J Neuroradiol Date: 2013-05-16 Impact factor: 3.825
Authors: Laurence O'Dwyer; Franck Lamberton; Arun L W Bokde; Michael Ewers; Yetunde O Faluyi; Colby Tanner; Bernard Mazoyer; Des O'Neill; Máiréad Bartley; D Rónán Collins; Tara Coughlan; David Prvulovic; Harald Hampel Journal: PLoS One Date: 2011-06-30 Impact factor: 3.240