Literature DB >> 21514328

Development of a pyrosequencing assay for rapid assessment of quinolone resistance in Acinetobacter baumannii isolates.

Yann Deccache1, Léonid M Irenge, Encho Savov, Mihaela Ariciuc, Alexandra Macovei, Angelina Trifonova, Ivanka Gergova, Jérôme Ambroise, Raymond Vanhoof, Jean-Luc Gala.   

Abstract

Rapid and reliable assessment of Acinetobacter baumannii resistance to quinolones was successfully achieved through pyrosequencing of the gyrA and parC quinolone-resistance determining regions. A strong correlation was found between quinolone resistance and mutations in gyrA codon 83 and/or in the parC gene (codons 80 or 84). Absence of QRDR mutations was associated with susceptibility to quinolones.
Copyright © 2011 Elsevier B.V. All rights reserved.

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Year:  2011        PMID: 21514328     DOI: 10.1016/j.mimet.2011.04.007

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN: 0167-7012            Impact factor:   2.363


  3 in total

1.  A Novel Mismatched PCR-Restriction Fragment Length Polymorphism Assay for Rapid Detection of gyrA and parC Mutations Associated With Fluoroquinolone Resistance in Acinetobacter baumannii.

Authors:  Naoki Kakuta; Ryuichi Nakano; Akiyo Nakano; Yuki Suzuki; Ayako Tanouchi; Takashi Masui; Saori Horiuchi; Shiro Endo; Risako Kakuta; Yasuo Ono; Hisakazu Yano
Journal:  Ann Lab Med       Date:  2020-01       Impact factor: 3.464

2.  A qPCR and multiplex pyrosequencing assay combined with automated data processing for rapid and unambiguous detection of ESBL-producers Enterobacteriaceae.

Authors:  Yann Deccache; Leonid M Irenge; Jérôme Ambroise; Encho Savov; Dan Marinescu; Raphael B Chirimwami; Jean-Luc Gala
Journal:  AMB Express       Date:  2015-08-12       Impact factor: 3.298

3.  AdvISER-PYRO: Amplicon Identification using SparsE Representation of PYROsequencing signal.

Authors:  Jérôme Ambroise; Anne-Sophie Piette; Cathy Delcorps; Leen Rigouts; Bouke C de Jong; Leonid Irenge; Annie Robert; Jean-Luc Gala
Journal:  Bioinformatics       Date:  2013-06-14       Impact factor: 6.937

  3 in total

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