| Literature DB >> 21513964 |
Choongho Lee1, Han Ma, Julie Qi Hang, Vincent Leveque, Ella H Sklan, Menashe Elazar, Klaus Klumpp, Jeffrey S Glenn.
Abstract
The hepatitis C virus (HCV) non-structural (NS) 5A protein plays an essential role in the replication of the viral RNA by the membrane-associated replication complex (RC). Recently, a putative NS5A inhibitor, BMS-790052, exhibited the highest potency of any known anti-HCV compound in inhibiting HCV replication in vitro and showed a promising clinical effect in HCV-infected patients. The precise mechanism of action for this new class of potential anti-HCV therapeutics, however, is still unclear. In order to gain further insight into its mode of action, we sought to test the hypothesis that the antiviral effect of BMS-790052 might be mediated by interfering with the functional assembly of the HCV RC. We observed that BMS-790052 indeed altered the subcellular localization and biochemical fractionation of NS5A. Taken together, our data suggest that NS5A inhibitors such as BMS-790052 can suppress viral genome replication by altering the proper localization of NS5A into functional RCs.Entities:
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Year: 2011 PMID： 21513964 PMCID： PMC3096933 DOI： 10.1016/j.virol.2011.03.026
Source DB: PubMed Journal: Virology ISSN： 0042-6822 Impact factor: 3.616