| Literature DB >> 21510941 |
Monika Dahiya1, Satish Singh, Govindan Rajamohan, Deepti Sethi, Kanak L Dikshit.
Abstract
Staphylokinase (SAK) forms a 1:1 stoichiometric complex with human plasmin (Pm) and switches its substrate specificity to generate a plasminogen (Pg) activator complex. Site-directed mutagenesis of SAKHis43 and SAKTyr44 demonstrated the crucial requirement of a positively charged and an aromatic residue, respectively, at these positions for optimal functioning of SAK-Pm activator complex. Molecular modeling studies further revealed the role of these residues in making cation-pi and pi-pi interactions with Trp215 of Pm and thus establishing the crucial intermolecular contacts within the active site cleft of the activator complex for the cofactor activity of SAK.Entities:
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Year: 2011 PMID： 21510941 DOI： 10.1016/j.febslet.2011.04.030
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124