| Literature DB >> 21507443 |
Zhaogang Sun1, Rui Cao, Miao Tian, Xuxia Zhang, Xiyue Zhang, Yan Li, Yuhui Xu, Weixing Fan, Baoxu Huang, Chuanyou Li.
Abstract
Mycobacterium bovis, the causative agent of bovine tuberculosis (bTB) in cattle, is also a pathogen for human and other mammals. In this study, 406 cows were screened for bTB by both single intradermal comparative cervical tuberculin (SICCT) test and IFN-γ assay. 135 M. bovis were isolated from 31 SICCT and IFN-γ double-positive cows in Xinjiang Uygur Autonomous Region of China. Spoligotyping and MIRU-VNTR were evaluated for genotyping, and 4 and 7 genotypes were identified, respectively. A new combination of nine MIRU-VNTR loci was most discriminative for M. bovis clones from Xinjiang. Interestingly, two new spoligotypes (SB1903 and SB1904) and special repeat numbers of three loci (ETR-D, QUB 1895 and QUB 3336) were discovered in this study. These results indicated a specific epidemic conservation in Xinjiang, China. M. bovis strains with the unique genotypes were isolated from the herds maintaining parent cows imported from the bTB-free countries, suggesting a possible transmission from the local breed of Xinjiang brown cattle.Entities:
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Year: 2011 PMID： 21507443 DOI： 10.1016/j.rvsc.2011.03.012
Source DB: PubMed Journal: Res Vet Sci ISSN： 0034-5288 Impact factor: 2.534