J Zhang1, C Xu, L Guo, B Ke, C Ke, B Zhang, X L Deng, M Liao. 1. Key Laboratory of Animal Disease Control and Prevention of the Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Abstract
AIMS: To develop a modified pulsed-field gel electrophoresis (PFGE) method for characterizing Haemophilus parasuis isolates. METHODS AND RESULTS: A modified PFGE procedure was designed using CpoI to generate restriction maps of H. parasuis genomic DNA. This approach was used to characterize 47 H. parasuis clinical isolates and 15 reference strains. All strains could be typed by this method, and the procedure was completed in 36 h. A total of 39 different PFGE patterns were identified among 47 epidemiologically unrelated clinical isolates. CONCLUSIONS: The modified PGFE described in this report efficiently characterized H. parasuis isolates. This method can be adopted for studying the epidemiology of Glässer's disease outbreaks in addition to differentiating and classifying previously untypeable H. parasuis isolates. SIGNIFICANCE AND IMPACT OF THE STUDY: The modified PFGE method described is a novel means of characterizing H. parasuis isolates. It is also a highly discriminatory molecular typing method (discriminatory index of 0.98) that can overcome the limitations of serotyping. No claim to Chinese Government works. Letters in Applied Microbiology
AIMS: To develop a modified pulsed-field gel electrophoresis (PFGE) method for characterizing Haemophilus parasuis isolates. METHODS AND RESULTS: A modified PFGE procedure was designed using CpoI to generate restriction maps of H. parasuis genomic DNA. This approach was used to characterize 47 H. parasuis clinical isolates and 15 reference strains. All strains could be typed by this method, and the procedure was completed in 36 h. A total of 39 different PFGE patterns were identified among 47 epidemiologically unrelated clinical isolates. CONCLUSIONS: The modified PGFE described in this report efficiently characterized H. parasuis isolates. This method can be adopted for studying the epidemiology of Glässer's disease outbreaks in addition to differentiating and classifying previously untypeable H. parasuis isolates. SIGNIFICANCE AND IMPACT OF THE STUDY: The modified PFGE method described is a novel means of characterizing H. parasuis isolates. It is also a highly discriminatory molecular typing method (discriminatory index of 0.98) that can overcome the limitations of serotyping. No claim to Chinese Government works. Letters in Applied Microbiology
Authors: Jianmin Zhang; Chenggang Xu; Lili Guo; Haiyan Shen; Xiaoling Deng; Changwen Ke; Bixia Ke; Bin Zhang; Ang Li; Tao Ren; Ming Liao Journal: Can J Vet Res Date: 2012-07 Impact factor: 1.310
Authors: Yufeng Li; Amy H Y Kwok; Jingwei Jiang; Yao Zou; Fangyuan Zheng; Pan Chen; Chengcai Hou; Frederick C Leung; Ping Jiang Journal: PLoS One Date: 2013-09-02 Impact factor: 3.240