Literature DB >> 2150658

Carboxyl-terminal deletion analysis of the Streptococcus mutans glucosyltransferase-I enzyme.

C Kato1, H K Kuramitsu.   

Abstract

Sequential deletion of the carboxyl-terminal amino acids (including the six direct repeating units) of the glucosyltransferase-I (GTF-I) enzyme of Streptococcus mutans revealed differential effects on sucrase and GTF activities. Removal of all but one repeating unit resulted in a truncated enzyme with significant sucrase activity but no detectable GTF activity. These results are compatible with the presence of two functional domains in the enzyme.

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Year:  1990        PMID： 2150658     DOI： 10.1016/0378-1097(90)90321-g

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  19 in total

1.  Molecular characterization of DSR-E, an alpha-1,2 linkage-synthesizing dextransucrase with two catalytic domains.

Authors:  Sophie Bozonnet; Marguerite Dols-Laffargue; Emeline Fabre; Sandra Pizzut; Magali Remaud-Simeon; Pierre Monsan; René-Marc Willemot
Journal:  J Bacteriol       Date:  2002-10       Impact factor: 3.490

2.  Ligand-binding properties of the carboxyl-terminal repeat domain of Streptococcus mutans glucan-binding protein A.

Authors:  W Haas; J A Banas
Journal:  J Bacteriol       Date:  2000-02       Impact factor: 3.490

3.  Evidence for a modular structure of the homologous repetitive C-terminal carbohydrate-binding sites of Clostridium difficile toxins and Streptococcus mutans glucosyltransferases.

Authors:  C von Eichel-Streiber; M Sauerborn; H K Kuramitsu
Journal:  J Bacteriol       Date:  1992-10       Impact factor: 3.490

4.  Role of C-terminal direct repeating units of the Streptococcus mutans glucosyltransferase-S in glucan binding.

Authors:  M Lis; T Shiroza; H K Kuramitsu
Journal:  Appl Environ Microbiol       Date:  1995-05       Impact factor: 4.792

5.  Identification of essential amino acids in the Streptococcus mutans glucosyltransferases.

Authors:  H Tsumori; T Minami; H K Kuramitsu
Journal:  J Bacteriol       Date:  1997-06       Impact factor: 3.490

6.  Effect of Leuconostoc mesenteroides NRRL B-512F dextransucrase carboxy-terminal deletions on dextran and oligosaccharide synthesis.

Authors:  V Monchois; A Reverte; M Remaud-Simeon; P Monsan; R M Willemot
Journal:  Appl Environ Microbiol       Date:  1998-05       Impact factor: 4.792

7.  Deletions in the carboxyl-terminal region of Streptococcus gordonii glucosyltransferase affect cell-associated enzyme activity and sucrose-associated accumulation of growing cells.

Authors:  M M Vickerman; D B Clewell
Journal:  Appl Environ Microbiol       Date:  1997-05       Impact factor: 4.792

8.  Mechanism of Streptococcus mutans glucosyltransferases: hybrid-enzyme analysis.

Authors:  Y J Nakano; H K Kuramitsu
Journal:  J Bacteriol       Date:  1992-09       Impact factor: 3.490

9.  Purification, characterization, and specificity of dextranase inhibitor (Dei) expressed from Streptococcus sobrinus UAB108 gene cloned in Escherichia coli.

Authors:  J W Sun; S Y Wanda; R Curtiss
Journal:  J Bacteriol       Date:  1995-04       Impact factor: 3.490

10.  Identification of amino acid residues in Streptococcus mutans glucosyltransferases influencing the structure of the glucan product.

Authors:  A Shimamura; Y J Nakano; H Mukasa; H K Kuramitsu
Journal:  J Bacteriol       Date:  1994-08       Impact factor: 3.490
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