| Literature DB >> 21505242 |
Fiona Cuskin1, Alexandra S Solovyova, Richard J Lewis, Paul R Race.
Abstract
The antibiotic bacillaene is biosynthesized in Bacillus subtilis by a hybrid type 1 modular polyketide synthase/nonribosomal peptide synthetase of the trans-acyltransferase (trans-AT) class. Within this system, the essential acyl-group loading activity is provided by the action of three free-standing trans-acting acyltransferases. Here, the recombinant expression, purification and crystallization of the bacillaene synthase trans-acting acyltransferase PksC are reported. A diffraction data set has been collected from a single PksC crystal to 1.44 Å resolution and the crystal was found to belong to the orthorhombic space group P2(1)2(1)2(1).Entities:
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Year: 2011 PMID： 21505242 PMCID： PMC3080151 DOI： 10.1107/S1744309111003484
Source DB: PubMed Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun ISSN： 1744-3091