Literature DB >> 21504768

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers.

Masaru Kuwayama1, Naoki Shigemoto, Sachiko Oohara, Yukie Tanizawa, Hiroko Yamada, Yoshihiro Takeda, Takeshi Matsuo, Shinji Fukuda.   

Abstract

We have developed simultaneous detection of eight genes associated with the five categories of diarrheagenic Escherichia coli by the multiplex PCR assay with Alexa Fluor-labeled primers. This assay can easily distinguish eight genes based on the size and color of amplified products without gel staining.
Copyright © 2011 Elsevier B.V. All rights reserved.

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Year:  2011        PMID: 21504768     DOI: 10.1016/j.mimet.2011.04.004

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN: 0167-7012            Impact factor:   2.363


  4 in total

1.  Development and accuracy of quantitative real-time polymerase chain reaction assays for detection and quantification of enterotoxigenic Escherichia coli (ETEC) heat labile and heat stable toxin genes in travelers' diarrhea samples.

Authors:  Bonnie P Youmans; Nadim J Ajami; Zhi-Dong Jiang; Joseph F Petrosino; Herbert L DuPont; Sarah K Highlander
Journal:  Am J Trop Med Hyg       Date:  2013-11-04       Impact factor: 2.345

2.  Universal Multiplex PCR: a novel method of simultaneous amplification of multiple DNA fragments.

Authors:  Daxing Wen; Chunqing Zhang
Journal:  Plant Methods       Date:  2012-08-15       Impact factor: 4.993

3.  Molecular Characterization of Diarrheagenic Escherichia Coli in Children Less Than 5 Years of Age with Diarrhea in Ouagadougou, Burkina Faso.

Authors:  Ali Konaté; René Dembélé; Assèta Kagambèga; Issiaka Soulama; Wendpoulomdé A D Kaboré; Emmanuel Sampo; Haoua Cissé; Antoine Sanou; Samuel Serme; Soumanaba Zongo; Cheikna Zongo; Alio Mahamadou Fody; Nathalie K Guessennd; Alfred S Traoré; Amy Gassama-Sow; Nicolas Barro
Journal:  Eur J Microbiol Immunol (Bp)       Date:  2017-08-09

4.  Detection and discrimination of five E. coli pathotypes using a combinatory SYBR® Green qPCR screening system.

Authors:  Elodie Barbau-Piednoir; Sarah Denayer; Nadine Botteldoorn; Katelijne Dierick; Sigrid C J De Keersmaecker; Nancy H Roosens
Journal:  Appl Microbiol Biotechnol       Date:  2018-02-20       Impact factor: 4.813

  4 in total

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