OBJECTIVE: We studied phosphorylation of insulin-receptors substrate downstream molecules: 1) in the ex-vivo visceral adipose tissue (VAT) of patients with aldosterone-producing adenoma (APA) (no.=7) and non-functioning adenoma (NFA) (no.=7) undergoing laparoscopic adrenalectomy; 2) in aldosterone-treated sc adipocytes of subjects (no.=5) who requested abdominoplasty. PATIENTS AND METHODS: Western blotting was used to detect phosphorylation of Akt and extracellular signal-regulated kinase (ERK) 1/2 in VAT from APA and NFA patients, and in subcutaneous adipocytes pre-treated with different aldosterone concentrations. Phosphorylation of Akt and ERK1/2 was similar in VAT of patients with APA and NFA. Pre-treatment in adipocytes with both physiological (1 nM) and pharmacological (10 μM) doses of aldosterone did not affect basal or insulin-induced phosphorylation of Akt and ERK1/2. CONCLUSIONS: Our data give further evidence that insulin signaling in human VAT is not affected by primary aldosterone overproduction.
OBJECTIVE: We studied phosphorylation of insulin-receptors substrate downstream molecules: 1) in the ex-vivo visceral adipose tissue (VAT) of patients with aldosterone-producing adenoma (APA) (no.=7) and non-functioning adenoma (NFA) (no.=7) undergoing laparoscopic adrenalectomy; 2) in aldosterone-treated sc adipocytes of subjects (no.=5) who requested abdominoplasty. PATIENTS AND METHODS: Western blotting was used to detect phosphorylation of Akt and extracellular signal-regulated kinase (ERK) 1/2 in VAT from APA and NFA patients, and in subcutaneous adipocytes pre-treated with different aldosterone concentrations. Phosphorylation of Akt and ERK1/2 was similar in VAT of patients with APA and NFA. Pre-treatment in adipocytes with both physiological (1 nM) and pharmacological (10 μM) doses of aldosterone did not affect basal or insulin-induced phosphorylation of Akt and ERK1/2. CONCLUSIONS: Our data give further evidence that insulin signaling in human VAT is not affected by primary aldosterone overproduction.
Authors: Francesco Fallo; Franco Veglio; Chiara Bertello; Nicoletta Sonino; Paolo Della Mea; Mario Ermani; Franco Rabbia; Giovanni Federspil; Paolo Mulatero Journal: J Clin Endocrinol Metab Date: 2005-11-15 Impact factor: 5.958
Authors: Gillian E Walker; Barbara Verti; Paolo Marzullo; Guilio Savia; Monica Mencarelli; Francesco Zurleni; Antonio Liuzzi; Anna Maria Di Blasio Journal: Obesity (Silver Spring) Date: 2007-08 Impact factor: 5.002
Authors: Laura L Gathercole; Iwona J Bujalska; Paul M Stewart; Jeremy W Tomlinson Journal: J Clin Endocrinol Metab Date: 2007-08-21 Impact factor: 5.958