BACKGROUND/AIMS: In oxidative stress, heme oxygenase-1 (HO-1) plays a pivotal role in maintaining renal function and protecting renal structure, especially in renal tubular epithelial cells. We examined urinary HO-1 (uHO-1) levels to assess whether uHO-1 acts as a sensitive biomarker for detecting tubulointerstitial inflammatory damage in renal diseases. METHODS: Immunohistochemical analyses and enzyme-linked immunosorbent assays for uHO-1 were performed using 61 urine samples (supernatants and sediment lysates) from healthy children and renal disease patients. RESULTS: Proximal and distal epithelial cells showed higher uHO-1 levels than squamous and urothelial cells. Inflammatory renal disease patients had higher uHO-1 levels than noninflammatory renal disease patients and controls. In IgA nephropathy, patients with interstitial cellular infiltration showed higher uHO-1 levels than those without it. Among patients with increased urinary β(2)-microglobulin or N-acetyl-β-D-glucosaminidase levels, uHO-1 levels increased only in those with renal disease and tubulointerstitial inflammatory damage. uHO-1 levels positively correlated with urinary interleukin-6 in inflammatory renal disease patients. CONCLUSIONS: These results indicate that uHO-1 is a potentially useful, novel, and noninvasive biomarker for evaluating the degree of tubulointerstitial inflammatory damage in renal disease.
BACKGROUND/AIMS: In oxidative stress, heme oxygenase-1 (HO-1) plays a pivotal role in maintaining renal function and protecting renal structure, especially in renal tubular epithelial cells. We examined urinary HO-1 (uHO-1) levels to assess whether uHO-1 acts as a sensitive biomarker for detecting tubulointerstitial inflammatory damage in renal diseases. METHODS: Immunohistochemical analyses and enzyme-linked immunosorbent assays for uHO-1 were performed using 61 urine samples (supernatants and sediment lysates) from healthy children and renal diseasepatients. RESULTS: Proximal and distal epithelial cells showed higher uHO-1 levels than squamous and urothelial cells. Inflammatory renal diseasepatients had higher uHO-1 levels than noninflammatory renal disease patients and controls. In IgA nephropathy, patients with interstitial cellular infiltration showed higher uHO-1 levels than those without it. Among patients with increased urinary β(2)-microglobulin or N-acetyl-β-D-glucosaminidase levels, uHO-1 levels increased only in those with renal disease and tubulointerstitial inflammatory damage. uHO-1 levels positively correlated with urinary interleukin-6 in inflammatory renal diseasepatients. CONCLUSIONS: These results indicate that uHO-1 is a potentially useful, novel, and noninvasive biomarker for evaluating the degree of tubulointerstitial inflammatory damage in renal disease.
Authors: Susanne Ramm; Petar Todorov; Vidya Chandrasekaran; Anders Dohlman; Maria B Monteiro; Mira Pavkovic; Jeremy Muhlich; Harish Shankaran; William W Chen; Jerome T Mettetal; Vishal S Vaidya Journal: Toxicol Sci Date: 2019-05-01 Impact factor: 4.849
Authors: Melanie Adler; Susanne Ramm; Marc Hafner; Jeremy L Muhlich; Esther Maria Gottwald; Elijah Weber; Alenka Jaklic; Amrendra Kumar Ajay; Daniel Svoboda; Scott Auerbach; Edward J Kelly; Jonathan Himmelfarb; Vishal S Vaidya Journal: J Am Soc Nephrol Date: 2015-08-10 Impact factor: 10.121
Authors: Ana Konvalinka; Joyce Zhou; Apostolos Dimitromanolakis; Andrei P Drabovich; Fei Fang; Susan Gurley; Thomas Coffman; Rohan John; Shao-Ling Zhang; Eleftherios P Diamandis; James W Scholey Journal: J Biol Chem Date: 2013-07-11 Impact factor: 5.157