BACKGROUND: Antithyroglobulin antibodies are a prevalent cause of interference in serum thyroglobulin immunoassays. Current guidelines recommend that antithyroglobulin antibodies should be measured concurrently with thyroglobulin when monitoring thyroid cancer patients post-thyroidectomy. However, the concordance between different antithyroglobulin assays has been questioned despite the availability of an international thyroglobulin antibody Reference Preparation. METHODS: Four antithyroglobulin assays currently in use in UK laboratories (Siemens Immulite(®), Brahms GmbH, PerkinElmer AutoDELFIA and Siemens ADVIA Centaur(®)) were compared in a cohort of 145 thyroid cancer patients. RESULTS: Using reference data provided by the kit manufacturer, concordance between the assays was 74%. Adjusting the cut-offs to maximize agreement increased concordance to 90%. Recovery of exogenous thyroglobulin using the Brahms Tg-plus immunoradiometric assay was neither a specific nor a sensitive test for the presence of a positive antibody result by any assay. CONCLUSIONS: Despite the availability of an international reference preparation, current antithyroglobulin assays show unacceptable variance.
BACKGROUND: Antithyroglobulin antibodies are a prevalent cause of interference in serum thyroglobulin immunoassays. Current guidelines recommend that antithyroglobulin antibodies should be measured concurrently with thyroglobulin when monitoring thyroid cancerpatients post-thyroidectomy. However, the concordance between different antithyroglobulin assays has been questioned despite the availability of an international thyroglobulin antibody Reference Preparation. METHODS: Four antithyroglobulin assays currently in use in UK laboratories (Siemens Immulite(®), Brahms GmbH, PerkinElmer AutoDELFIA and Siemens ADVIA Centaur(®)) were compared in a cohort of 145 thyroid cancerpatients. RESULTS: Using reference data provided by the kit manufacturer, concordance between the assays was 74%. Adjusting the cut-offs to maximize agreement increased concordance to 90%. Recovery of exogenous thyroglobulin using the Brahms Tg-plus immunoradiometric assay was neither a specific nor a sensitive test for the presence of a positive antibody result by any assay. CONCLUSIONS: Despite the availability of an international reference preparation, current antithyroglobulin assays show unacceptable variance.
Authors: Bryan R Haugen; Erik K Alexander; Keith C Bible; Gerard M Doherty; Susan J Mandel; Yuri E Nikiforov; Furio Pacini; Gregory W Randolph; Anna M Sawka; Martin Schlumberger; Kathryn G Schuff; Steven I Sherman; Julie Ann Sosa; David L Steward; R Michael Tuttle; Leonard Wartofsky Journal: Thyroid Date: 2016-01 Impact factor: 6.568
Authors: Alessandra De Remigis; Tanja D de Gruijl; Jennifer N Uram; Schey-Cherng Tzou; Shintaro Iwama; Monica V Talor; Todd D Armstrong; Saskia J A M Santegoets; Susan F Slovin; Lei Zheng; Daniel A Laheru; Elizabeth M Jaffee; Winald R Gerritsen; Alfons J M van den Eertwegh; Dung T Le; Patrizio Caturegli Journal: Int J Cancer Date: 2014-05-28 Impact factor: 7.396
Authors: F D'Aurizio; P Metus; A Ferrari; B Caruso; R Castello; D Villalta; A Steffan; K Gaspardo; F Pesente; N Bizzaro; E Tonutti; S Valverde; C Cosma; M Plebani; R Tozzoli Journal: Auto Immun Highlights Date: 2017-06-19
Authors: Germán A Jimenez Londoño; Ana Maria Garcia Vicente; Julia Sastre Marcos; Francisco Jose Pena Pardo; Mariano Amo-Salas; Manuel Moreno Caballero; Maria Prado Talavera Rubio; Beatriz Gonzalez Garcia; Niletys Dafne Disotuar Ruiz; Angel Maria Soriano Castrejón Journal: Eur Thyroid J Date: 2018-07-05