BACKGROUND AND OBJECTIVES: We tagged melanoma cells with gold nanoparticles to show their viability for increasing sensitivity in a photoacoustic detection system. Ultimately, this study models the detection of circulating tumor cells, which are an important prognostic factor in the progress of melanoma. STUDY DESIGN/ MATERIALS AND METHODS: A Q-switched, tunable Nd:YAG laser was used to irradiate cells in both a stationary and flow set-up. Photoacoustic signals were measured using a polyvinylidene fluoride (PVDF) film in the stationary test, and a commercially available ultrasonic probe for flow tests. Both unmodified melanoma cells and gold nanoparticle (AuNP) tagged melanoma were tested. RESULTS: AuNP tagged melanoma in a stationary set-up showed an average of 0.227 mV/mJ larger signal than the untagged, indicating a signal increase of 34%. At 500 nm there is a maximum difference of 0.295 mV/mJ, or a 41% increase. In flow tests, the ultrasound probe was able to detect single cells, but the increased signal from AuNP tagging was minimal. CONCLUSION: AuNP tagging proved to give an increased photoacoustic signal allowing greater sensitivity in stationary metastasized melanoma detection systems using photoacoustics.
BACKGROUND AND OBJECTIVES: We tagged melanoma cells with gold nanoparticles to show their viability for increasing sensitivity in a photoacoustic detection system. Ultimately, this study models the detection of circulating tumor cells, which are an important prognostic factor in the progress of melanoma. STUDY DESIGN/ MATERIALS AND METHODS: A Q-switched, tunable Nd:YAG laser was used to irradiate cells in both a stationary and flow set-up. Photoacoustic signals were measured using a polyvinylidene fluoride (PVDF) film in the stationary test, and a commercially available ultrasonic probe for flow tests. Both unmodified melanoma cells and gold nanoparticle (AuNP) tagged melanoma were tested. RESULTS:AuNP tagged melanoma in a stationary set-up showed an average of 0.227 mV/mJ larger signal than the untagged, indicating a signal increase of 34%. At 500 nm there is a maximum difference of 0.295 mV/mJ, or a 41% increase. In flow tests, the ultrasound probe was able to detect single cells, but the increased signal from AuNP tagging was minimal. CONCLUSION:AuNP tagging proved to give an increased photoacoustic signal allowing greater sensitivity in stationary metastasized melanoma detection systems using photoacoustics.
Authors: Benjamin S Goldschmidt; Smit Mehta; Jeff Mosley; Chris Walter; Paul J D Whiteside; Heather K Hunt; John A Viator Journal: Biomed Opt Express Date: 2013-10-14 Impact factor: 3.732
Authors: Christine M O'Brien; Kyle D Rood; Kiran Bhattacharyya; Thiago DeSouza; Shramik Sengupta; Sagar K Gupta; Jeffrey D Mosley; Benjamin S Goldschmidt; Nikhilesh Sharma; John A Viator Journal: J Biomed Opt Date: 2012-06 Impact factor: 3.170
Authors: Benjamin S Goldschmidt; Anna M Rudy; Charissa A Nowak; Yowting Tsay; Paul J D Whiteside; Heather K Hunt Journal: J Vis Exp Date: 2016-07-26 Impact factor: 1.355