| Literature DB >> 21499749 |
Yuelan Zhao1, Chengmin Wang, Yanmin Lu, Said Amer, Ping Xu, Jianyong Wang, Junxia Lu, Yongzhan Bao, Bolin Deng, Hongxuan He, Jianhua Qin.
Abstract
Eimeria acervulina was isolated from chicken at Hebei province, China. The gene of merozoite surface antigen 3-1E was amplified and cloned into pET28a(+) vector and then transformed into Escherichia coli BL21 strain. Results showed that 3-1E fusion protein band of about 22 kDa was identified by SDS-PAGE. Western blot analysis indicated that the recombinant protein specifically reacted with E. acervulina polyclonal antibody.Entities:
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Year: 2011 PMID: 21499749 DOI: 10.1007/s00436-011-2381-9
Source DB: PubMed Journal: Parasitol Res ISSN: 0932-0113 Impact factor: 2.289