OBJECTIVE: (1) Evaluate the presence of biofilms in patients with chronic sinusitis with nasal polyps (CRSwNP) and (2) investigate the association of biofilm presence and CRSwNP. STUDY DESIGN: Cross-sectional study. SETTING: University-based tertiary care center. SUBJECTS AND METHODS: The study group consisted of 33 consecutive patients undergoing functional endoscopic sinus surgery for CRSwNP. The control group consisted of 27 control patients undergoing septoplasty for nasal obstruction without diagnosis of chronic sinusitis. Mucosal samples were harvested intraoperatively for scanning electron microscopic examination to determine biofilm presence. Statistical analysis was performed. For all statistical tests, P = .05 was considered significant. RESULTS: Biofilms were found in 24 (72.7%) of the 33 patients with CRSwNP and in 13 (48.1%) of the 27 septoplasty patients (odds ratio = 2.87; 95% confidence interval, 0.98-8.42; P = .051). CONCLUSION: (1) Biofilms were present in patients undergoing functional endoscopic sinus surgery for CRSwNP and also in controls without chronic sinusitis. This suggests that biofilms may not be sufficient to cause chronic sinusitis without other cofactors. Host factors could be the responsible for the pathogenesis of biofilms. (2) Although the prevalence of biofilms in patients with CRSwNP was not significantly different from that in the controls, the extremely wide 95% confidence interval, which is just below unity, suggests that a meaningful clinical difference may have been missed because of low statistical power. Further studies are necessary.
OBJECTIVE: (1) Evaluate the presence of biofilms in patients with chronic sinusitis with nasal polyps (CRSwNP) and (2) investigate the association of biofilm presence and CRSwNP. STUDY DESIGN: Cross-sectional study. SETTING: University-based tertiary care center. SUBJECTS AND METHODS: The study group consisted of 33 consecutive patients undergoing functional endoscopic sinus surgery for CRSwNP. The control group consisted of 27 control patients undergoing septoplasty for nasal obstruction without diagnosis of chronic sinusitis. Mucosal samples were harvested intraoperatively for scanning electron microscopic examination to determine biofilm presence. Statistical analysis was performed. For all statistical tests, P = .05 was considered significant. RESULTS: Biofilms were found in 24 (72.7%) of the 33 patients with CRSwNP and in 13 (48.1%) of the 27 septoplasty patients (odds ratio = 2.87; 95% confidence interval, 0.98-8.42; P = .051). CONCLUSION: (1) Biofilms were present in patients undergoing functional endoscopic sinus surgery for CRSwNP and also in controls without chronic sinusitis. This suggests that biofilms may not be sufficient to cause chronic sinusitis without other cofactors. Host factors could be the responsible for the pathogenesis of biofilms. (2) Although the prevalence of biofilms in patients with CRSwNP was not significantly different from that in the controls, the extremely wide 95% confidence interval, which is just below unity, suggests that a meaningful clinical difference may have been missed because of low statistical power. Further studies are necessary.
Authors: Rogério Pezato; Leonardo Balsalobre; Milena Lima; Thiago F P Bezerra; Richard L Voegels; Luis Carlos Gregório; Aldo Cassol Stamm; Thibaut van Zele Journal: J Otolaryngol Head Neck Surg Date: 2013-03-28
Authors: Rogério Pezato; Luciano Bottura; Rodrigo de Paula Santos; Richard Louis Voegels; Andre Luis Lacerda Bachi; Luis Carlos Gregório Journal: J Otolaryngol Head Neck Surg Date: 2013-07-19
Authors: Judd H Fastenberg; Wayne D Hsueh; Ali Mustafa; Nadeem A Akbar; Waleed M Abuzeid Journal: World J Otorhinolaryngol Head Neck Surg Date: 2016-05-05
Authors: Joshua P Earl; Nithin D Adappa; Jaroslaw Krol; Archana S Bhat; Sergey Balashov; Rachel L Ehrlich; James N Palmer; Alan D Workman; Mariel Blasetti; Bhaswati Sen; Jocelyn Hammond; Noam A Cohen; Garth D Ehrlich; Joshua Chang Mell Journal: Microbiome Date: 2018-10-23 Impact factor: 14.650