Literature DB >> 21491986

The fluorescence theatre: a cost-effective device using theatre gels for fluorescent protein and dye screening.

John R Heil1, Ricardo F Nordeste, Trevor C Charles.   

Abstract

Here we report a simple cost-effective device for screening colonies on plates for expression of the monomeric red fluorescent protein mRFP1 and the fluorescent dye Nile red. This device can be built from any simple light source, in our case a Quebec Colony Counter, and cost-effective theatre gels. The device can be assembled in as little as 20 min, and it produces excellent results when screening a large number of colonies.

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Year:  2011        PMID: 21491986     DOI: 10.1139/w11-010

Source DB:  PubMed          Journal:  Can J Microbiol        ISSN: 0008-4166            Impact factor:   2.419


  2 in total

1.  Site-specific bacterial chromosome engineering: ΦC31 integrase mediated cassette exchange (IMCE).

Authors:  John R Heil; Jiujun Cheng; Trevor C Charles
Journal:  J Vis Exp       Date:  2012-03-16       Impact factor: 1.355

2.  Lactic acid containing polymers produced in engineered Sinorhizobium meliloti and Pseudomonas putida.

Authors:  Tam T Tran; Trevor C Charles
Journal:  PLoS One       Date:  2020-03-19       Impact factor: 3.240

  2 in total

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