Literature DB >> 21490687

Peptide affinity reagents for AAV capsid recognition and purification.

N Pulicherla1, A Asokan.   

Abstract

We report the discovery of AAV capsid-binding peptides identified through phage panning. The heptapeptide motif GYVSRHP selectively recognized AAV serotype 8 capsids and blocked transduction in vitro. Recombinant AAV8 vectors were purified directly from crude cell lysate and supernatant through sequential application of peptide affinity and anion exchange chromatography. Peptide affinity reagents may serve as useful alternatives to monoclonal antibodies in AAV capsid recognition, and offer readily scalable solutions for purification of clinical grade AAV vectors.

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Year:  2011        PMID： 21490687      PMCID： PMC3192935          DOI： 10.1038/gt.2011.46

Source DB:  PubMed          Journal:  Gene Ther        ISSN： 0969-7128            Impact factor:   5.250


  20 in total

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Authors:  Gregory S Arnold; A Kate Sasser; Matthew D Stachler; Jeffrey S Bartlett
Journal:  Mol Ther       Date:  2006-04-19       Impact factor: 11.454

3.  Efficient serotype-dependent release of functional vector into the culture medium during adeno-associated virus manufacturing.

Authors:  Luk H Vandenberghe; Ru Xiao; Martin Lock; Jianping Lin; Michael Korn; James M Wilson
Journal:  Hum Gene Ther       Date:  2010-10       Impact factor: 5.695

Review 4.  Critical assessment of current adeno-associated viral vector production and quantification methods.

Authors:  Marc G Aucoin; Michel Perrier; Amine A Kamen
Journal:  Biotechnol Adv       Date:  2007-09-08       Impact factor: 14.227

Review 5.  Evolution of phage display: from bioactive peptides to bioselective nanomaterials.

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6.  Cross-packaging of a single adeno-associated virus (AAV) type 2 vector genome into multiple AAV serotypes enables transduction with broad specificity.

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7.  Production and characterization of adeno-associated viral vectors.

Authors:  Joshua C Grieger; Vivian W Choi; R Jude Samulski
Journal:  Nat Protoc       Date:  2006       Impact factor: 13.491

8.  Engineering adeno-associated virus for one-step purification via immobilized metal affinity chromatography.

Authors:  James T Koerber; Jae-Hyung Jang; Julie H Yu; Ravi S Kane; David V Schaffer
Journal:  Hum Gene Ther       Date:  2007-04       Impact factor: 5.695

9.  Chromatography-based purification of adeno-associated virus.

Authors:  Richard H Smith; Linda Yang; Robert M Kotin
Journal:  Methods Mol Biol       Date:  2008

Review 10.  Display technologies: application for the discovery of drug and gene delivery agents.

Authors:  Anna Sergeeva; Mikhail G Kolonin; Jeffrey J Molldrem; Renata Pasqualini; Wadih Arap
Journal:  Adv Drug Deliv Rev       Date:  2006-10-06       Impact factor: 15.470
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  3 in total

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Journal:  J Phys Chem B       Date:  2017-02-14       Impact factor: 2.991

Review 2.  Scalable downstream strategies for purification of recombinant adeno- associated virus vectors in light of the properties.

Authors:  Weihong Qu; Mingxi Wang; Yaqing Wu; Ruian Xu
Journal:  Curr Pharm Biotechnol       Date:  2015       Impact factor: 2.837

3.  Targeting photoreceptors via intravitreal delivery using novel, capsid-mutated AAV vectors.

Authors:  Christine N Kay; Renee C Ryals; George V Aslanidi; Seok Hong Min; Qing Ruan; Jingfen Sun; Frank M Dyka; Daniel Kasuga; Andrea E Ayala; Kim Van Vliet; Mavis Agbandje-McKenna; William W Hauswirth; Sanford L Boye; Shannon E Boye
Journal:  PLoS One       Date:  2013-04-26       Impact factor: 3.240
  3 in total

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