Literature DB >> 21490185

Identification of Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa in blood cultures: a multicenter performance evaluation of a three-color peptide nucleic acid fluorescence in situ hybridization assay.

Phyllis Della-Latta1, Hossein Salimnia, Theresa Painter, Fann Wu, Gary W Procop, Deborah A Wilson, Wendy Gillespie, Alayna Mender, Benjamin S Crystal.   

Abstract

A multicenter evaluation was undertaken to evaluate the performance of a new three-color peptide nucleic acid fluorescence in situ hybridization assay that identifies isolates directly from blood cultures positive for Gram-negative bacilli (GNB). The assay correctly identified 100% (186/186) of the Escherichia coli isolates, 99.1% (109/110) of the Klebsiella pneumoniae isolates, and 95.8% (46/48) of the Pseudomonas aeruginosa isolates in this study. Negative assay results were correctly obtained for 162 of 165 other GNB (specificity, 98.2%).

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Year:  2011        PMID： 21490185      PMCID： PMC3122727          DOI： 10.1128/JCM.02250-10

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  9 in total

1.  Impact of rapid in situ hybridization testing on coagulase-negative staphylococci positive blood cultures.

Authors:  Graeme N Forrest; Sanjay Mehta; Elizabeth Weekes; Durry P Lincalis; Jennifer K Johnson; Richard A Venezia
Journal:  J Antimicrob Chemother       Date:  2006-04-24       Impact factor: 5.790

2.  Pseudomonas aeruginosa bacteremia: risk factors for mortality and influence of delayed receipt of effective antimicrobial therapy on clinical outcome.

Authors:  Cheol-In Kang; Sung-Han Kim; Hong-Bin Kim; Sang-Won Park; Young-Ju Choe; Myoung-Don Oh; Eui-Chong Kim; Kang-Won Choe
Journal:  Clin Infect Dis       Date:  2003-08-23       Impact factor: 9.079

3.  Multicenter evaluation of a new shortened peptide nucleic acid fluorescence in situ hybridization procedure for species identification of select Gram-negative bacilli from blood cultures.

Authors:  Margie Morgan; Elizabeth Marlowe; Phyllis Della-Latta; Hossein Salimnia; Susan Novak-Weekley; Fann Wu; Benjamin S Crystal
Journal:  J Clin Microbiol       Date:  2010-03-31       Impact factor: 5.948

4.  Pseudomonas aeruginosa bloodstream infection: importance of appropriate initial antimicrobial treatment.

Authors:  Scott T Micek; Ann E Lloyd; David J Ritchie; Richard M Reichley; Victoria J Fraser; Marin H Kollef
Journal:  Antimicrob Agents Chemother       Date:  2005-04       Impact factor: 5.191

5.  Peptide nucleic acid fluorescence in situ hybridization-based identification of Candida albicans and its impact on mortality and antifungal therapy costs.

Authors:  G N Forrest; K Mankes; M A Jabra-Rizk; E Weekes; J K Johnson; D P Lincalis; R A Venezia
Journal:  J Clin Microbiol       Date:  2006-09       Impact factor: 5.948

6.  Bloodstream infections caused by antibiotic-resistant gram-negative bacilli: risk factors for mortality and impact of inappropriate initial antimicrobial therapy on outcome.

Authors:  Cheol-In Kang; Sung-Han Kim; Wan Beom Park; Ki-Deok Lee; Hong-Bin Kim; Eui-Chong Kim; Myoung-Don Oh; Kang-Won Choe
Journal:  Antimicrob Agents Chemother       Date:  2005-02       Impact factor: 5.191

7.  Overview of nosocomial infections caused by gram-negative bacilli.

Authors:  Robert Gaynes; Jonathan R Edwards
Journal:  Clin Infect Dis       Date:  2005-08-16       Impact factor: 9.079

8.  Cost savings with implementation of PNA FISH testing for identification of Candida albicans in blood cultures.

Authors:  Barbara D Alexander; Elizabeth Dodds Ashley; L Barth Reller; Shelby D Reed
Journal:  Diagn Microbiol Infect Dis       Date:  2006-02-08       Impact factor: 2.803

9.  Prevalence and antimicrobial susceptibilities of bacteria isolated from blood cultures of hospitalized patients in the United States in 2002.

Authors:  James A Karlowsky; Mark E Jones; Deborah C Draghi; Clyde Thornsberry; Daniel F Sahm; Gregory A Volturo
Journal:  Ann Clin Microbiol Antimicrob       Date:  2004-05-10       Impact factor: 3.944
  9 in total
  5 in total

1.  ID Learning Unit-Diagnostics Update: Current Laboratory Methods for Rapid Pathogen Identification in Patients With Bloodstream Infections.

Authors:  Matthew P Rubach; Kimberly E Hanson
Journal:  Open Forum Infect Dis       Date:  2015-11-12       Impact factor: 3.835

2.  Evaluation of FISH for Blood Cultures under Diagnostic Real-Life Conditions.

Authors:  Annalena Reitz; Sven Poppert; Melanie Rieker; Hagen Frickmann
Journal:  Eur J Microbiol Immunol (Bp)       Date:  2018-12-12

3.  Efficacy of single versus multiple exposure by electromagnetic modalities on gram-negative and positive bacterial strains in an in-vitro model.

Authors:  Snehil Dixit; Irfan Ahmad; Kumar Gular; Refaat A Eid; Ravi Shankar Reddy; Ivana Leão Ribeiro; Mohammed Abohashrh; Mastour Saeed Alshahrani; Jaya Shanker Tedla; Nitin Arun Dixit
Journal:  Saudi J Biol Sci       Date:  2020-12-08       Impact factor: 4.219

4.  Fluorescence In Situ Hybridization (FISH) and Peptide Nucleic Acid Probe-Based FISH for Diagnosis of Q Fever Endocarditis and Vascular Infections.

Authors:  Elsa Prudent; Hubert Lepidi; Emmanouil Angelakis; Didier Raoult
Journal:  J Clin Microbiol       Date:  2018-08-27       Impact factor: 11.677

Review 5.  Diagnostic Molecular Microbiology: A 2018 Snapshot.

Authors:  Marilynn Ransom Fairfax; Martin H Bluth; Hossein Salimnia
Journal:  Clin Lab Med       Date:  2018-06       Impact factor: 1.935
  5 in total

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