Literature DB >> 21486060

Facile method of constructing polyproteins for single-molecule force spectroscopy studies.

Peng Zheng1, Yi Cao, Hongbin Li.   

Abstract

Constructing polyproteins consisting of identical tandem repeats of proteins provides an unambiguous method of investigating the mechanical properties of proteins at the single-molecule level using force spectroscopy techniques. Here we report a maleimide-thiol coupling-based facile method of constructing polyproteins for single-molecule force spectroscopy studies on the mechanical properties of proteins. This method allows for the construction of polyproteins in an efficient fashion under room temperature. The resultant thioether bonds are resistant to reduction and make it possible to carry out single-molecule force spectroscopy studies under various redox conditions. This novel method complements existing polyprotein engineering methods and can be easily applied to a wide variety of proteins.

Entities:  

Mesh：

Substances：

Year:  2011        PMID： 21486060     DOI： 10.1021/la200915d

Source DB:  PubMed          Journal:  Langmuir        ISSN： 0743-7463            Impact factor:   3.882


  10 in total

1.  Protein misfolding occurs by slow diffusion across multiple barriers in a rough energy landscape.

Authors:  Hao Yu; Derek R Dee; Xia Liu; Angela M Brigley; Iveta Sosova; Michael T Woodside
Journal:  Proc Natl Acad Sci U S A       Date:  2015-06-24       Impact factor: 11.205

2.  The molecular mechanism underlying mechanical anisotropy of the protein GB1.

Authors:  Yongnan Devin Li; Guillaume Lamour; Jörg Gsponer; Peng Zheng; Hongbin Li
Journal:  Biophys J       Date:  2012-12-05       Impact factor: 4.033

3.  Direct measurements of the mechanical stability of zinc-thiolate bonds in rubredoxin by single-molecule atomic force microscopy.

Authors:  Peng Zheng; Hongbin Li
Journal:  Biophys J       Date:  2011-09-20       Impact factor: 4.033

4.  Mechanically untying a protein slipknot: multiple pathways revealed by force spectroscopy and steered molecular dynamics simulations.

Authors:  Chengzhi He; Georgi Z Genchev; Hui Lu; Hongbin Li
Journal:  J Am Chem Soc       Date:  2012-06-15       Impact factor: 15.419

5.  Invincible DNA tethers: covalent DNA anchoring for enhanced temporal and force stability in magnetic tweezers experiments.

Authors:  Richard Janissen; Bojk A Berghuis; David Dulin; Max Wink; Theo van Laar; Nynke H Dekker
Journal:  Nucleic Acids Res       Date:  2014-08-19       Impact factor: 16.971

6.  Detection of weak non-covalent cation-π interactions in NGAL by single-molecule force spectroscopy.

Authors:  Jingyuan Nie; Yibing Deng; Fang Tian; Shengchao Shi; Peng Zheng
Journal:  Nano Res       Date:  2022-01-11       Impact factor: 10.269

7.  Tailored Polyproteins Using Sequential Staple and Cut.

Authors:  Surbhi Garg; Gayathri Sindhuri Singaraju; Sunanda Yengkhom; Sabyasachi Rakshit
Journal:  Bioconjug Chem       Date:  2018-04-30       Impact factor: 4.774

8.  Force-induced chemical reactions on the metal centre in a single metalloprotein molecule.

Authors:  Peng Zheng; Guilherme M Arantes; Martin J Field; Hongbin Li
Journal:  Nat Commun       Date:  2015-06-25       Impact factor: 14.919

9.  Enzymatic biosynthesis and immobilization of polyprotein verified at the single-molecule level.

Authors:  Yibing Deng; Tao Wu; Mengdi Wang; Shengchao Shi; Guodong Yuan; Xi Li; Hanchung Chong; Bin Wu; Peng Zheng
Journal:  Nat Commun       Date:  2019-06-24       Impact factor: 14.919

10.  Engineering protein polymers of ultrahigh molecular weight via supramolecular polymerization: towards mimicking the giant muscle protein titin.

Authors:  Ruidi Wang; Jiayu Li; Xiumei Li; Jin Guo; Junqiu Liu; Hongbin Li
Journal:  Chem Sci       Date:  2019-08-20       Impact factor: 9.825
  10 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.