BACKGROUND: Human leukocyte antigen G (HLA-G) is a non-classical Ib molecule in the major histocompatibility complex. HLA-G has important immunosuppressive properties, and in the context of cardiac transplantation, is associated with a low risk of cellular rejection. A 14-bp insertion/deletion polymorphism in exon 8 of the HLA-G gene is associated with messenger RNA (mRNA) stability and expression of HLA-G. This study analyzed the relationship between HLA-G polymorphisms and serum HLA-G levels in patients after cardiac transplantation to determine if any specific HLA-G genotype is associated with cellular rejection. METHODS: Ninety-four heart transplant patients were genotyped for the 14-bp polymorphism. Serum HLA-G levels and cellular rejection grades were evaluated in all patients. RESULTS: The 14-bp polymorphism was significantly associated with serum HLA-G expression. Patients with the -14-bp/-14-bp genotype had significantly higher mean serum HLA-G levels (88.2 U/ml) than those patients with the +14-bp/-14-bp (52.8 U/ml) and +14-bp/+14-bp (32.2 U/ml) genotypes (p = 0.004). The -14 bp/-14-bp genotype was significantly associated with fewer episodes of cellular rejection. CONCLUSIONS: This study suggests that the 14-bp deletion in the HLA-G gene plays an important role in the expression of HLA-G and thus might be a clinically useful genetic indicator for cellular rejection risk after cardiac transplantation.
BACKGROUND:Human leukocyte antigen G (HLA-G) is a non-classical Ib molecule in the major histocompatibility complex. HLA-G has important immunosuppressive properties, and in the context of cardiac transplantation, is associated with a low risk of cellular rejection. A 14-bp insertion/deletion polymorphism in exon 8 of the HLA-G gene is associated with messenger RNA (mRNA) stability and expression of HLA-G. This study analyzed the relationship between HLA-G polymorphisms and serum HLA-G levels in patients after cardiac transplantation to determine if any specific HLA-G genotype is associated with cellular rejection. METHODS: Ninety-four heart transplant patients were genotyped for the 14-bp polymorphism. Serum HLA-G levels and cellular rejection grades were evaluated in all patients. RESULTS: The 14-bp polymorphism was significantly associated with serum HLA-G expression. Patients with the -14-bp/-14-bp genotype had significantly higher mean serum HLA-G levels (88.2 U/ml) than those patients with the +14-bp/-14-bp (52.8 U/ml) and +14-bp/+14-bp (32.2 U/ml) genotypes (p = 0.004). The -14 bp/-14-bp genotype was significantly associated with fewer episodes of cellular rejection. CONCLUSIONS: This study suggests that the 14-bp deletion in the HLA-G gene plays an important role in the expression of HLA-G and thus might be a clinically useful genetic indicator for cellular rejection risk after cardiac transplantation.
Authors: H P V Silva; M A G Ururahy; K S C Souza; M B Loureiro; Y M C Oliveira; G H M Oliveira; A D Luchessi; K T C Carvalho; J C O C Freitas; E A Donadi; R D C Hirata; M G Almeida; R F Arrais; M H Hirata; A A Rezende Journal: Genes Immun Date: 2015-10-22 Impact factor: 2.676
Authors: Siqi Liu; Nicolaas A Bos; Erik A M Verschuuren; Debbie van Baarle; Johanna Westra Journal: Front Immunol Date: 2022-06-13 Impact factor: 8.786
Authors: Erick C Castelli; Jaqueline Ramalho; Iane O P Porto; Thálitta H A Lima; Leandro P Felício; Audrey Sabbagh; Eduardo A Donadi; Celso T Mendes-Junior Journal: Front Immunol Date: 2014-10-06 Impact factor: 7.561
Authors: Moniek H C Craenmehr; Geert W Haasnoot; Jos J M Drabbels; Marijke J Spruyt-Gerritse; Milo Cao; Carin van der Keur; Johanna M Kapsenberg; Merve Uyar-Mercankaya; Els van Beelen; Tess Meuleman; Marie-Louise P van der Hoorn; Sebastiaan Heidt; Frans H J Claas; Michael Eikmans Journal: HLA Date: 2019-08-08 Impact factor: 4.513