| Literature DB >> 21468974 |
Benjamin Gantenbein-Ritter1, Christoph M Sprecher, Samantha Chan, Svenja Illien-Jünger, Sibylle Grad.
Abstract
In tissue engineering, a variety of methods are commonly used to evaluate survival of cells inside tissues or three-dimensional (3D) carriers. Among these methods confocal laser scanning microscopy opened accessibility of 3D tissue using live cell imaging into the tissue or 3D scaffolds. However, although this technique is ideally applied to 3D tissue or scaffolds with thickness up to several millimetres, this application is surprisingly rare and scans are often done on slices with thickness <20 μm. Here, we present novel protocols for the staining of 3D tissue (e.g. intervertebral disc tissue) and scaffolds, such as fibrin gels or alginate beads.Entities:
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Year: 2011 PMID： 21468974 DOI： 10.1007/978-1-61779-108-6_14
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745