Literature DB >> 21468963

Estimation of cell number based on metabolic activity: the MTT reduction assay.

László Kupcsik1.   

Abstract

The MTT reduction assay is used to determine the level of metabolic activity in eukaryotic cells, -including animal, plant, and fungal cells. If the metabolic rate is constant, the technique can be employed to count living cells in a sample. Once it is set up, the method is very robust, and can be automatized to be applied on a large number of samples.

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Year:  2011        PMID: 21468963     DOI: 10.1007/978-1-61779-108-6_3

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  22 in total

1.  Optimizing Targeted Inhibitors of P-Glycoprotein Using Computational and Structure-Guided Approaches.

Authors:  John G Wise; Amila K Nanayakkara; Maha Aljowni; Gang Chen; Maisa C De Oliveira; Lauren Ammerman; Ketetha Olengue; Alexander R Lippert; Pia D Vogel
Journal:  J Med Chem       Date:  2019-11-26       Impact factor: 7.446

2.  Toward understanding the role of cartilage particulates in synovial inflammation.

Authors:  A M Silverstein; R M Stefani; E Sobczak; E L Tong; M G Attur; R P Shah; J C Bulinski; G A Ateshian; C T Hung
Journal:  Osteoarthritis Cartilage       Date:  2017-03-30       Impact factor: 6.576

3.  Cationic branched polymers for cellular delivery of negatively charged cargo.

Authors:  Courtney A Follit; Shannon R Woodruff; Pia D Vogel; John G Wise; Nicolay V Tsarevsky
Journal:  J Drug Deliv Sci Technol       Date:  2017-04-07       Impact factor: 3.981

4.  Cytotoxic action of methylquercetins in human lung adenocarcinoma cells.

Authors:  Katrin Sak; Helen Lust; Marju Kase; Jana Jaal
Journal:  Oncol Lett       Date:  2017-11-21       Impact factor: 2.967

5.  [Non-antagonistic influence of Krumeich's intrastromal corneal ring in an experimental tissue culture system].

Authors:  C Schmidt; S Fabinyi; S Rehfeldt; S Klöpzig; V Jentzen; J Bohrisch; A Messner; J Storsberg
Journal:  Ophthalmologe       Date:  2016-10       Impact factor: 1.059

6.  Mitochondria-specific accumulation of amyloid β induces mitochondrial dysfunction leading to apoptotic cell death.

Authors:  Moon-Yong Cha; Sun-Ho Han; Sung Min Son; Hyun-Seok Hong; Young-Ju Choi; Jayoung Byun; Inhee Mook-Jung
Journal:  PLoS One       Date:  2012-04-13       Impact factor: 3.240

7.  Natural mineral particles are cytotoxic to rainbow trout gill epithelial cells in vitro.

Authors:  Christian Michel; Simon Herzog; Christian de Capitani; Patricia Burkhardt-Holm; Constanze Pietsch
Journal:  PLoS One       Date:  2014-07-03       Impact factor: 3.240

8.  Pilot-Scale Pyrolytic Remediation of Crude-Oil-Contaminated Soil in a Continuously-Fed Reactor: Treatment Intensity Trade-Offs.

Authors:  Wen Song; Julia E Vidonish; Roopa Kamath; Pingfeng Yu; Chun Chu; Bhagavatula Moorthy; Baoyu Gao; Kyriacos Zygourakis; Pedro J J Alvarez
Journal:  Environ Sci Technol       Date:  2019-02-08       Impact factor: 9.028

9.  Long term and standard incubations of WST-1 reagent reflect the same inhibitory trend of cell viability in rat airway smooth muscle cells.

Authors:  Lei-Miao Yin; Yin Wei; Yu Wang; Yu-Dong Xu; Yong-Qing Yang
Journal:  Int J Med Sci       Date:  2012-12-13       Impact factor: 3.738

10.  Diabetic levels of glucose increase cellular reducing equivalents but reduce survival in three models of 661W photoreceptor-like cell injury.

Authors:  Christopher J Layton
Journal:  BMC Ophthalmol       Date:  2015-12-09       Impact factor: 2.209

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