BACKGROUND: A clear-cut need exists for safe and effective alternatives to the use of isotretinoin in severe acne. Lack of data regarding the specifics of isotretinoin's mechanism of action has hampered progress in this area. Recently, the protein neutrophil gelatinase-associated lipocalin (NGAL) has been identified as a mediator of the apoptotic effect of isotretinoin on sebocytes. OBJECTIVES: To establish further the clinical relevance of NGAL and to elucidate the factors that induce NGAL expression in sebocytes. METHODS: Methods were developed to isolate and quantify skin-surface levels of NGAL from normal subjects and patients with acne undergoing treatment with isotretinoin. RESULTS: Patients with acne were found to have higher skin levels of NGAL compared with normal subjects. Studies in SEB-1 sebocytes indicate that NGAL expression is increased in response to Propionibacterium acnes and interleukin (IL)-1β. In patients, isotretinoin increases NGAL levels by 2·4-fold on the skin surface and this increase precedes decreases in sebum and P. acnes counts. CONCLUSIONS: These data support the hypothesis that NGAL is an important mediator of the early effects of isotretinoin on the sebaceous glands and provide insights into the mechanisms that regulate NGAL expression in the skin.
BACKGROUND: A clear-cut need exists for safe and effective alternatives to the use of isotretinoin in severe acne. Lack of data regarding the specifics of isotretinoin's mechanism of action has hampered progress in this area. Recently, the protein neutrophil gelatinase-associated lipocalin (NGAL) has been identified as a mediator of the apoptotic effect of isotretinoin on sebocytes. OBJECTIVES: To establish further the clinical relevance of NGAL and to elucidate the factors that induce NGAL expression in sebocytes. METHODS: Methods were developed to isolate and quantify skin-surface levels of NGAL from normal subjects and patients with acne undergoing treatment with isotretinoin. RESULTS:Patients with acne were found to have higher skin levels of NGAL compared with normal subjects. Studies in SEB-1 sebocytes indicate that NGAL expression is increased in response to Propionibacterium acnes and interleukin (IL)-1β. In patients, isotretinoin increases NGAL levels by 2·4-fold on the skin surface and this increase precedes decreases in sebum and P. acnes counts. CONCLUSIONS: These data support the hypothesis that NGAL is an important mediator of the early effects of isotretinoin on the sebaceous glands and provide insights into the mechanisms that regulate NGAL expression in the skin.
Authors: G L Peck; T G Olsen; F W Yoder; J S Strauss; D T Downing; M Pandya; D Butkus; J Arnaud-Battandier Journal: N Engl J Med Date: 1979-02-15 Impact factor: 91.245
Authors: David H Goetz; Margaret A Holmes; Niels Borregaard; Martin E Bluhm; Kenneth N Raymond; Roland K Strong Journal: Mol Cell Date: 2002-11 Impact factor: 17.970
Authors: Guanhu Bao; Matthew Clifton; Trisha M Hoette; Kiyoshi Mori; Shi-Xian Deng; Andong Qiu; Melanie Viltard; David Williams; Neal Paragas; Thomas Leete; Ritwij Kulkarni; Xiangpo Li; Belinda Lee; Avtandil Kalandadze; Adam J Ratner; Juan Carlos Pizarro; Kai M Schmidt-Ott; Donald W Landry; Kenneth N Raymond; Roland K Strong; Jonathan Barasch Journal: Nat Chem Biol Date: 2010-06-27 Impact factor: 15.040
Authors: Glen Lindwall; Elaine A Hsieh; Lisa M Misell; Christine M Chai; Scott M Turner; Marc K Hellerstein Journal: J Invest Dermatol Date: 2006-04 Impact factor: 8.551
Authors: Hanna-Leena Kelhälä; Riitta Palatsi; Nanna Fyhrquist; Sari Lehtimäki; Juha P Väyrynen; Matti Kallioinen; Minna E Kubin; Dario Greco; Kaisa Tasanen; Harri Alenius; Beatrice Bertino; Isabelle Carlavan; Bruno Mehul; Sophie Déret; Pascale Reiniche; Philippe Martel; Carine Marty; Ulrike Blume-Peytavi; Johannes J Voegel; Antti Lauerma Journal: PLoS One Date: 2014-08-25 Impact factor: 3.240