Literature DB >> 21464598

A RAPD-PCR method for the rapid detection of Bacillus cereus.

Jiyeon Lee1, Gun-Hee Kwon, Jae-Yong Park, Cheon-Seok Park, Dae Young Kwon, Jinkyu Lim, Jong Sang Kim, Jeong Hwan Kim.   

Abstract

Distinction of Bacillus cereus from other closely related bacilli is challenging and new efficient methods are continually demanded. From our previous work on RAPD profiles of bacilli, we found a possibility that B. cereus strains could be distinguished from other bacilli. In this work, RAPD-PCR profiles of B. cereus strains were obtained using a 10-mer (S30) as a primer, and a B. cereus specific 0.91-kb band was produced from all tested strains. The RAPD-PCR procedure also successfully detected B. cereus from spiked cheonggukjang when B. cereus cells were present at more than 10(2)/g sample.

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Year:  2011        PMID: 21464598

Source DB:  PubMed          Journal:  J Microbiol Biotechnol        ISSN: 1017-7825            Impact factor:   2.351


  2 in total

1.  Genetic diversity of Brazilian Bacillus thuringiensis isolates with toxicity against Aedes aegypti (Diptera: Culicidae).

Authors:  Geysla da Costa Fernandes; Dalton Kaynnan de Prado Costa; Nayanne Santos de Oliveira; Emanuelle Cristine Pereira de Sousa; Déborah Heloísa Bittencourt Machado; Ricardo Antonio Polanczyk; Herbert Álvaro Abreu de Siqueira; Maria Cleoneide da Silva
Journal:  Sci Rep       Date:  2022-08-24       Impact factor: 4.996

2.  Frequency of hemolysin BL and non-hemolytic enterotoxin complex genes of Bacillus cereus in raw and cooked meat samples in Zanjan, Iran.

Authors:  Habib Zeighami; Gholamreza Nejad-Dost; Angineh Parsadanians; Shahrzad Daneshamouz; Fakhri Haghi
Journal:  Toxicol Rep       Date:  2019-12-23
  2 in total

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