ETHNOPHARMACOLOGICAL RELEVANCE: Sheng-Ma-Ge-Gen-Tang (SMGGT; Shoma-kakkon-to) has been used against pediatric viral infection for thousands of year in ancient China. However, it is unknown whether SMGGT is effective against human respiratory syncytial virus (HRSV). AIM OF THE STUDY: HRSV is a major pediatric viral pathogen of low respiratory tract infection without effective management. This study tested the hypothesis that SMGGT effectively inhibited cytopathy induced by HRSV. MATERIALS AND METHODS: Effect of the crude extract of SMGGT on HRSV was tested by plaque reduction assay in both human upper (HEp-2) and low (A549) respiratory tract cell lines. Ability of SMGGT to stimulate anti-viral cytokines was evaluated by enzyme-linked immunosorbent assay (ELISA). RESULTS: Crude extract of SMGGT dose-dependently inhibited HRSV-induced plaque formation. The crude extract was more effective when given before viral infection (p<0.0001). It inhibited viral attachment dose-dependently (p<0.0001) and could increase heparin effect on viral attachment. Furthermore, it was synergistic with very low-dose heparin on viral attachment. In addition, the crude extract time-dependently and dose-dependently (p<0.0001) inhibited HRSV internalization into HEp-2 cells. Epithelial cells secrete IFN-β and TNF-α to counteract viral infection. The crude extract could stimulate epithelial cells to secrete these cytokines beforehand and become resistant to viral infection. It also stimulated IFN-β to defense HRSV after viral inoculation. CONCLUSIONS: Sheng-Ma-Ge-Gen-Tang could be effective to manage HRSV infection in young children.
ETHNOPHARMACOLOGICAL RELEVANCE: Sheng-Ma-Ge-Gen-Tang (SMGGT; Shoma-kakkon-to) has been used against pediatric viral infection for thousands of year in ancient China. However, it is unknown whether SMGGT is effective against human respiratory syncytial virus (HRSV). AIM OF THE STUDY: HRSV is a major pediatric viral pathogen of low respiratory tract infection without effective management. This study tested the hypothesis that SMGGT effectively inhibited cytopathy induced by HRSV. MATERIALS AND METHODS: Effect of the crude extract of SMGGT on HRSV was tested by plaque reduction assay in both human upper (HEp-2) and low (A549) respiratory tract cell lines. Ability of SMGGT to stimulate anti-viral cytokines was evaluated by enzyme-linked immunosorbent assay (ELISA). RESULTS: Crude extract of SMGGT dose-dependently inhibited HRSV-induced plaque formation. The crude extract was more effective when given before viral infection (p<0.0001). It inhibited viral attachment dose-dependently (p<0.0001) and could increase heparin effect on viral attachment. Furthermore, it was synergistic with very low-dose heparin on viral attachment. In addition, the crude extract time-dependently and dose-dependently (p<0.0001) inhibited HRSV internalization into HEp-2 cells. Epithelial cells secrete IFN-β and TNF-α to counteract viral infection. The crude extract could stimulate epithelial cells to secrete these cytokines beforehand and become resistant to viral infection. It also stimulated IFN-β to defense HRSV after viral inoculation. CONCLUSIONS: Sheng-Ma-Ge-Gen-Tang could be effective to manage HRSV infection in young children.
Authors: Min Kyoung Kim; Chae Young Bang; Gwang Jun Yun; Hyang-Yu Kim; Young Pyo Jang; Se Young Choung Journal: BMC Complement Altern Med Date: 2016-04-07 Impact factor: 3.659
Authors: Li Li Lin; Jin Jun Shan; Tong Xie; Jian Ya Xu; Cun Si Shen; Liu Qing Di; Jia Bin Chen; Shou Chuan Wang Journal: Evid Based Complement Alternat Med Date: 2016-09-04 Impact factor: 2.629
Authors: Chia Feng Yeh; Kuo Chih Wang; Lien Chai Chiang; Den En Shieh; Ming Hong Yen; Jung San Chang Journal: J Ethnopharmacol Date: 2013-04-30 Impact factor: 4.360