| Literature DB >> 21462864 |
Meiling Yao1, Xingxiao Zhang, Jing Gao, Tongjie Chai, Zengmin Miao, Weiming Ma, Mei Qin, Qinglei Li, Xiaoxia Li, Jingbo Liu, Hongshuang Zhang.
Abstract
To better understand the transmission route of H9N2 avian influenza virus (AIV), two duplicate trials were conducted to observe the process of aerosol infection and direct contact in specific pathogen free chickens. Fifteen chickens (G1) were inoculated with H9N2 AIV and housed together with another 15 chickens (G2) in the same positive-negative-pressure isolator (A). Fifteen chickens (G3) were bred in another isolator (B) which was connected with A so that air could flow unidirectionally from A to B. Air, oropharyngeal and cloacal swabs, and blood samples were collected for the detection of aerosolized virus, virus shedding, and seroconversion. AIV aerosols were initially detected at day 2-3 post inoculation (dpi), reaching peak concentrations at 7 dpi. Virus shedding was detected in all chickens of G2, but only in a part in G3 (T1: 87%, T2: 80%). Antibodies were initially detected at 4-5 dpi, peaking at 14-21 dpi. The results showed that H9N2 AIV could be transmitted by both aerosol exposure and direct contact.Entities:
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Year: 2011 PMID： 21462864
Source DB: PubMed Journal: Berl Munch Tierarztl Wochenschr ISSN： 0005-9366 Impact factor: 0.328