Literature DB >> 21461674

Role of the all1549 (ana-rsh) gene, a relA/spoT homolog, of the Cyanobacterium Anabaena sp. PCC7120.

Degang Ning1, Yaru Qian, Xiaogang Miao, Chongwei Wen.   

Abstract

The role of a single relA/spoT homolog all1549 (designated hereafter as ana-rsh) of the cyanobacterium Anabaena sp. PCC7120 was investigated. The complementation test in Escherichia coli showed that the protein encoded by ana-rsh possesses guanosine tetraphosphate (p)ppGpp-synthase/hydrolase activity. Under laboratory growth conditions, a low level of ppGpp was detected in Anabaena sp. PCC7120 and the loss of ana-rsh was lethal. Amino acid starvation induced ppGpp accumulation to an appropriate level, and nitrogen deficiency did not alter the ppGpp concentration in Anabaena cells. These data suggest that ana-rsh is required for cell viability under normal growth conditions and involved in the (p)ppGpp-related stringent response to amino acid deprivation, but not related to heterocyst formation and nitrogen fixation of Anabaena sp. PCC7120.

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Year:  2011        PMID： 21461674     DOI： 10.1007/s00284-011-9926-0

Source DB:  PubMed          Journal:  Curr Microbiol        ISSN： 0343-8651            Impact factor:   2.188


  15 in total

1.  A RelA-SpoT homolog (Cr-RSH) identified in Chlamydomonas reinhardtii generates stringent factor in vivo and localizes to chloroplasts in vitro.

Authors:  Koji Kasai; Syoji Usami; Takashi Yamada; Yaeta Endo; Kozo Ochi; Yuzuru Tozawa
Journal:  Nucleic Acids Res       Date:  2002-11-15       Impact factor: 16.971

2.  Residual guanosine 3',5'-bispyrophosphate synthetic activity of relA null mutants can be eliminated by spoT null mutations.

Authors:  H Xiao; M Kalman; K Ikehara; S Zemel; G Glaser; M Cashel
Journal:  J Biol Chem       Date:  1991-03-25       Impact factor: 5.157

3.  Regulation by hetC of genes required for heterocyst differentiation and cell division in Anabaena sp. strain PCC 7120.

Authors:  Yu Wang; Xudong Xu
Journal:  J Bacteriol       Date:  2005-12       Impact factor: 3.490

Review 4.  (p)ppGpp: still magical?

Authors:  Katarzyna Potrykus; Michael Cashel
Journal:  Annu Rev Microbiol       Date:  2008       Impact factor: 15.500

5.  Conjugal transfer of DNA to cyanobacteria.

Authors:  J Elhai; C P Wolk
Journal:  Methods Enzymol       Date:  1988       Impact factor: 1.600

6.  Role of the (p)ppGpp synthase RSH, a RelA/SpoT homolog, in stringent response and virulence of Staphylococcus aureus.

Authors:  Tobias Geiger; Christiane Goerke; Michaela Fritz; Tina Schäfer; Knut Ohlsen; Manuel Liebeke; Michael Lalk; Christiane Wolz
Journal:  Infect Immun       Date:  2010-03-08       Impact factor: 3.441

7.  A versatile class of positive-selection vectors based on the nonviability of palindrome-containing plasmids that allows cloning into long polylinkers.

Authors:  J Elhai; C P Wolk
Journal:  Gene       Date:  1988-08-15       Impact factor: 3.688

8.  Conformational antagonism between opposing active sites in a bifunctional RelA/SpoT homolog modulates (p)ppGpp metabolism during the stringent response [corrected].

Authors:  Tanis Hogg; Undine Mechold; Horst Malke; Mike Cashel; Rolf Hilgenfeld
Journal:  Cell       Date:  2004-04-02       Impact factor: 41.582

9.  Mutational analysis of the Escherichia coli spoT gene identifies distinct but overlapping regions involved in ppGpp synthesis and degradation.

Authors:  D R Gentry; M Cashel
Journal:  Mol Microbiol       Date:  1996-03       Impact factor: 3.501

10.  Arabidopsis RelA/SpoT homologs implicate (p)ppGpp in plant signaling.

Authors:  E A van der Biezen; J Sun; M J Coleman; M J Bibb; J D Jones
Journal:  Proc Natl Acad Sci U S A       Date:  2000-03-28       Impact factor: 11.205
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  3 in total

1.  ppGpp metabolism is involved in heterocyst development in the cyanobacterium Anabaena sp. strain PCC 7120.

Authors:  Shao-Ran Zhang; Gui-Ming Lin; Wen-Li Chen; Li Wang; Cheng-Cai Zhang
Journal:  J Bacteriol       Date:  2013-08-09       Impact factor: 3.490

Review 2.  Survival strategies in the aquatic and terrestrial world: the impact of second messengers on cyanobacterial processes.

Authors:  Marco Agostoni; Beronda L Montgomery
Journal:  Life (Basel)       Date:  2014-11-18

Review 3.  Within and beyond the stringent response-RSH and (p)ppGpp in plants.

Authors:  Justyna Boniecka; Justyna Prusińska; Grażyna B Dąbrowska; Anna Goc
Journal:  Planta       Date:  2017-09-25       Impact factor: 4.116
  3 in total

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