Long-term Clinical Outcome and MIBI SPECT Parameters in Percutaneous Coronary Interventions.

BACKGROUND AND AIM: Primary percutaneous coronary intervention (PCI) is the preferred treatment option for acute myocardial infarction (MI). Off-site PCI reduces time-to-treatment, which could potentially lead to enhanced clinical outcomes. Therefore, we investigated whether off-site PCI improves 5-year clinical outcomes compared with on-site PCI and whether this is related to in-hospital (99m)Tc-sestamibi single photon emission computed tomography (MIBI SPECT) parameters.
METHODS: We describe the 5-year follow-up for a combined endpoint of death or re-infarction in 128 patients with acute MI who were randomly assigned to undergo primary PCI at the off-site centre (n = 68) or to transferral to an on-site centre (n = 60). Three days after PCI, MIBI SPECT was performed to estimate infarct size. A multivariate Cox regression model was created to study the relation between MIBI SPECT parameters and long-term clinical outcomes.
RESULTS: After a mean follow-up of 5.8 ± 1.1 years, 25 events occurred. Off-site PCI significantly reduced door-to-balloon time compared with on-site PCI (94 ± 54 versus 125 ± 59 min, p = 0.003). However, infarct size (17 ± 15 versus 14 ± 12%, p = 0.34) and 5-year death or infarct rate (21% versus 18%, p = 0.75) were comparable between treatment centres. With multivariate analysis, only Killip class ≥2 and Q wave MI, but not scintigraphic data, predicted long-term clinical outcomes.
CONCLUSION: Off-site PCI reduced door-to-balloon time with a comparable 5-year death or infarct rate. Parameters from resting MIBI SPECT on day 3 after MI did not predict long-term clinical outcomes.

RCT Entities:   Population Interventions Outcomes

Introduction

Primary percutaneous coronary intervention (PCI) is widely accepted as the preferred reperfusion strategy to reduce infarct size in patients with acute ST-segment elevation myocardial infarction (STEMI) [1]. Trials comparing PCI in STEMI patients presented to large community hospitals without on-site cardiac surgical backup (off-site) versus PCI in a tertiary interventional centre (on-site) have demonstrated that off-site PCI is feasible and results in comparable short-term clinical outcome [2,3]. Treatment in off-site centres results in a significant reduction in time-to-treatment, which could potentially lead to a reduction in infarct size by more timely salvage of acutely jeopardised ischaemic myocardium [4-6]. In that respect, off-site PCI without transfer delay in STEMI patients might even prove to be superior in limiting infarct size [7,8].

We extended our earlier findings [9] and investigated whether off-site PCI improves 5-year clinical outcomes compared with on-site PCI and if this is related to in-hospital 99mTc-sestamibi single photon emission computed tomography (SPECT) parameters.

Methods

Patients

This study describes the 5-year follow-up of STEMI patients who, in a previous study, were randomly assigned to primary PCI at the centre of presentation in a community hospital (off-site, n = 103) or to transferral to a remote tertiary centre with surgical backup facilities (on-site, n = 96) [6]. The in-hospital outcomes in relation to 99mTc-sestamibi SPECT parameters in 128 patients were described previously [9]. STEMI patients who presented within 6 h after the onset of symptoms, who were able to give informed consent and did not require mechanical ventilatory assistance were eligible for randomisation. Upon arrival at the cardiac care unit and prior to randomisation, all patients were immediately treated with heparin, aspirin, nitroglycerin, and abciximab, unless contraindicated. Standard treatment after the PCI procedure included clopidogrel, β-blockers, aspirin, ACE inhibitors, and statins, unless contraindicated. The protocol was approved by the review board or ethics committee of each hospital.

Of the original consecutive patient population (n = 199), 128 patients consented to 99mTc-sestamibi SPECT imaging 3 days after the cardiac event. Failure to undergo 99mTc-sestamibi SPECT was due to early death (5), emergency CABG (1), previous myocardial infarction (MI; 11), unable or unwilling to provide consent (42), no PCI was performed and the absence of MI (4).

Scintigraphy

Rest gated myocardial perfusion images were acquired approximately 1 h after an intravenous injection of 700 MBq 99mTc-sestamibi using SPECT. A dual-head camera system, equipped with low-energy high-resolution collimators, was used for myocardial imaging (Millennium VG; GE Healthcare). Images were acquired in a 64 × 64 matrix with an acquisition time of 30 s per image. The SPECT images were gated with eight frames per cardiac cycle, and the RR time acceptance was set at 20%. Transaxial slices were reconstructed via back projection with a ramp filter, followed by a butterworth filter. Using commercially available software, polar maps were created of the relative distribution of tracer uptake throughout the entire left ventricle (LV). Each polar map was normalised to its individual maximum, and the defect size was defined as <50% uptake area of the polar map and was subsequently expressed as a percentage of the LV.

Follow-up

Information on survival status was obtained in all patients in June 2009. Digital hospital records were reviewed for all-cause mortality and enzymatically confirmed myocardial re-infarction (re-MI), i.e. typical chest pain plus creatine kinase-MB (CK-MB) or troponin I above the upper limit of normal, i.e. >16 U/l and >0.45 μg/l, respectively.

Statistical Analysis

Data are expressed as mean ± standard deviation (SD). Frequencies were compared using Fisher’s exact test and Student’s t test, and continuous variables were compared using the independent t test. Comparison of multiple datasets was performed using ANOVA, and specific differences were identified by a Student’s t test corrected for multiple comparisons with the Bonferroni adjustment.

The incidence of the combined endpoint over time was evaluated according to the Kaplan–Meier method, and differences between groups of patients were analysed with the log-rank test. Univariate analysis of the combined endpoint over time for baseline and 99mTc-sestamibi SPECT parameters was performed using Cox proportional hazard regression. Variables with a p value <0.5 in univariate analysis were included in the multivariable model so that no (measured) confounders were missed, using the enter method. All analyses were performed using SPSS 14 (SPSS Inc., Chicago, Illinois). A p value <0.05 was considered significant.

Results

The study cohort consisted of 128 patients, 93 (73%) were men, and the mean age was 62 ± 12 SD years. Baseline characteristics and risk profile were comparable between the 60 patients randomised to on-site and 68 patients randomised to off-site PCI (Table 1). Mean follow-up was 5.8 years ± 1.1 SD. In this time frame, 15 re-MIs were documented, and ten patients died. Kaplan–Meier analysis demonstrated that there was no difference in long-term outcomes and centre of randomisation, log rank 0.715 (Fig. 1).

Table 1

Patient characteristics

	On-site (n = 60)	Off-site (n = 68)	p
Age (years)	64 ± 11	61 ± 12	0.62
Men	44 (73%)	49 (72%)	0.87
Diabetes mellitus	4 (7%)	2 (3%)	0.32
Smoker	24 (40%)	33 (49%)	0.59
Hypertension	15 (25%)	20 (29%)	0.58
Family history of CVD	27 (45%)	28 (41%)	0.66
Previous PCI	2 (3%)	2 (3%)	0.90
Previous CABG	3 (5%)	2 (3%)	0.55
Killip class >1	1 (1%)	1 (2%)	0.92
Summed ST deviation (mm)	15 ± 12	17 ± 9	0.27
Anterior myocardial infarction	23 (38%)	26 (38%)	0.99
Q wave	32 (53%)	42 (62%)	0.24
Multi (>1) vessel disease	23 (38%)	28 (41%)	0.74
TIMI flow pre PCI <2	38 (67%)	51 (75%)	0.31
TIMI flow post PCI <2	1 (2%)	2 (3%)	0.67
Symptoms-to-admission (min)	158 ± 131	163 ± 201	0.87
Symptoms-to-balloon (min)	286 ± 146	257 ± 211	0.38
Symptoms-to-arrival (min)	158 ± 131	163 ± 201	0.87
Door-to-balloon (min)	125 ± 59	94 ± 54	0.003
Time-to-treatment (min)	286 ± 146	257 ± 211	0.39

CVD cardiovascular disease, PCI percutaneous coronary intervention, CABG coronary artery bypass grafting, TIMI thrombolysis in myocardial infarction

Fig. 1

Difference in long-term follow-up between centres for the combined endpoint of either death or re-infarction; log rank, 0.715

Time to Treatment and Centre of Randomisation

The time lines revealed no difference in mean time from onset of symptoms to presentation at the clinic between groups. However, with a reduction of 31 min, the door-to-balloon time was significantly shorter in the off-site centre, although this did not result in a significant reduction in pain onset time-to-treatment. Neither did this reduction of door-to-balloon time result in an enzymatically smaller infarct size in off-site-treated patients, peak CK-MB 204 vs. 161 U/l; p value, 0.11 (Table 2). In univariate and multivariate analyses, this was further confirmed as none of the time line variables had a significant relation with long-term clinical outcomes.

Table 2

Comparison of centres regarding MIBI SPECT data and 5-year clinical outcomes

	On-site (n = 60)	Off-site (n = 68)	p
Death or infarct	11 (18%)	14 (21%)	0.75
LVESV (ml)	58 ± 26	65 ± 31	0.17
LVEDV (ml)	118 ± 36	124 ± 38	0.34
LVEF (%)	52 ± 12	50 ± 11	0.06
Infarct extent (%)	14 ± 12	17 ± 15	0.34
SRS	11 ± 9.6	12 ± 12	0.33
SMS	19 ± 13	24 ± 20	0.06
STS	14 ± 12	17 ± 15	0.028
Peak CK	1611 ± 1838	1894 ± 1609	0.35
Peak CK-MB (U/l)	161 ± 136	204 ± 160	0.11

Continuous data are presented as mean values ± standard deviation. Dichotomous data are presented as numbers and percentages

SPECT single photon emission computed tomography, LVESV left ventricular end-systolic volume, LVEDV left ventricular end-diastolic volume, LVEF left ventricular ejection fraction, SRS summed rest score, SMS summed motion score, STS summed thickening score, CK creatine kinase

Scintigraphic Data and Centre of Randomisation

Various scintigraphic variables were analysed, but most were comparable between on- and off-site PCI. However, the summed thickening score (STS) was smaller in patients randomised to on-site treatment, 14 ± 12 versus 17 ± 15 SD; p value, 0.028 (Table 2).

Scintigraphic Data and 5-Year Clinical Outcome

We investigated if parameters from a 99mTc-sestamibi SPECT on day 3 were related to the combined endpoint of death or re-MI at 5-year follow-up. However, in univariate and multivariate Cox regression analyses, none of the assessed scintigraphic parameters were related to this combined endpoint. Only a higher Killip class and Q wave infarction were related (Table 3).

Table 3

Univariate and multivariate analyses for the long-term combined endpoint (death/re-MI)

	Crude hazard ratio and 95% CI	Multivariate adjusted hazard ratio and 95% CI	P value
Off-site centre	1.2 (0.53–2.6)	1.3 (0.56–3.2)	0.51
Age (years)	1.03 (0.99–1.06)	1.03 (0.99–1.1)	0.14
Men	1.0 (0.42–2.4)	–
Diabetes mellitus	3.2 (0.97–11)	2.5 (0.63–9.7)	0.20
Smoker	1.3 (0.72–2.2)	1.3 (0.74–2.3)	0.36
Hypertension	1.3 (0.54–2.9)	–
Family history of CVD	0.60 (0.26–1.4)	0.76 (0.31–1.8)	0.54
Previous PCI	0.05 (0–600)	–
Previous CABG	1.1 (0.15–8.3)	–
Killip class >1	2.8 (0.38–21)	25.1 (2.0–315)	0.013
Summed ST deviation (mm)	1.03 (0.72–1.5)	–
Anterior myocardial infarction	1.3 (0.60–2.9)	1.4 (0.49–4.0)	0.54
Q wave	3.1 (1.2–8.4)	3.4 (1.02–11.2)	0.046
Multi-vessel disease (>1)	1.1 (0.49–2.4)	–
TIMI flow pre PCI <2	1.2 (0.51–2.8)	–
TIMI flow post PCI <2	0.46 (0.06–3.4)	0.75 (0.08–7.3)	0.80
Symptoms-to-admission (h)	1.06 (0.96–1.16)	0.94 (0.61–1.5)	0.79
Door-to-balloon (h)	1.01 (0.68–1.5)	–
Time-to-treatment (h)	1.05 (0.96–1.15)	1.09 (0.71–1.7)	0.69
LVESV (10 ml)	1.02 (0.90–1.2)	–
LVEDV (10 ml)	1.01 (0.91–1.1)	–
LVEF (%)	1.0 (0.97–1.03)	–
Infarct extent (%)	1.02 (0.99–1.04)	1.0 (0.82–1.2)	0.97
SRS	1.02 (0.99–1.05)	1.03 (0.80–1.3)	0.83
SMS	1.01 (0.99–1.03)	1.03 (0.95–1.1)	0.50
STS	1.01 (0.99–1.03)	0.95 (0.86–1.1)	0.35

Variables with a p value <0.5 in univariate analysis and all scintigraphic variables were included in the multivariable model

MI myocardial infarction, LVESV left ventricular end-systolic volume, LVEDV left ventricular end-diastolic volume, LVEF left ventricular ejection fraction, SRS summed rest score, SMS summed motion score, STS summed thickening score

Discussion

The results indicate that despite a 31-minute reduction of door-to-balloon time, scintigraphic parameters and 5-year clinical outcome are not considerably affected by performing off-site PCI. Additionally, in our series, off-site PCI is equally safe compared with on-site PCI. However, the reduction in door-to-balloon time did not result in improved 5-year clinical outcomes with off-site PCI.

Scintigraphic Data and Long-Term Clinical Outcome

Scintigraphic parameters did not predict 5-year clinical outcomes. This is in contrast to the results of Spinelli et al. who found that dysfunctional but viable myocardium with preserved systolic thickening was the strongest predictor of cardiovascular risk [10]. However, the infarct size in their study was quite small, while larger infarcts, i.e. >12% of LV myocardial mass correlate to mortality [11]. Clinical factors that independently predicted 5-year clinical outcomes in our series were a higher Killip class and Q-wave myocardial infarction.

On-Site Versus Off-Site PCI: Influence on Clinical and Scintigraphic Parameters

In spite of a reduced door-to-balloon time, off-site PCI did not result in a significantly different infarct size or long-term clinical outcomes, although the STS was smaller in on-site PCI. However, the 31-min reduction in door-to-balloon time in off-site PCI was relatively short. Myocardial salvage is particularly pronounced within the first 2 h of infarction and levels off in the ensuing hours [12]. Given the average time-to-treatment of 4.5 h, this may have obscured a potential benefit, particularly as few patients (10%) were treated within this early timeframe.

Door-to-Balloon Time: Influence on Clinical and Scintigraphic Parameters

Earlier large studies demonstrated that time-to-treatment is important in salvaging acutely jeopardised myocardium when performing a primary PCI [5,13]. However, in our study, a relationship between timing of reperfusion and infarct size or 5-year clinical outcome could not be established. This is similar to results from smaller studies that provided conflicting results with the larger studies [6,14].

Limitations

The number of investigated patients was relatively small and may explain why the impact of time on infarct size or long-term clinical outcome was not apparent. In addition, this study was performed in an area with a good infrastructure and relatively small inter-hospital transfer distances. In a more remote area, differences might actually become apparent.

The 99mTc-sestamibi SPECT might have been too early, since early cardiac remodelling after the acute phase takes several weeks before a final equilibrium has been reached [15]. Therefore, it might have been more appropriate to perform this scan at a later stage, e.g. 6 weeks after the event.

Conclusion

After a mean follow-up of 5.8 years, long-term clinical outcomes and scintigraphic parameters were not affected by performing off-site PCI, despite a 31-min reduction of door-to-balloon time. Parameters from rest gated 99mTc-sestamibi SPECT on day 3 after MI did not predict long-term clinical outcomes.