Literature DB >> 21460115

Overestimation of the abundance of sulfate-reducing bacteria in human feces by quantitative PCR targeting the Desulfovibrio 16S rRNA gene.

C T Christophersen1, M Morrison, M A Conlon.   

Abstract

The dominant genus of sulfate-reducing bacteria (SRB) in humans is Desulfovibrio, and quantitative PCR (QPCR) targeting the 16S rRNA gene is often used in assays. We show that the 16S rRNA gene assay overestimated SRB abundance in feces from 24 adults compared to QPCR assays using primers targeting two genes involved in SRB energy metabolism.

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Year:  2011        PMID： 21460115      PMCID： PMC3126453          DOI： 10.1128/AEM.02851-10

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  25 in total

1.  Antagonistic effects of sulfide and butyrate on proliferation of colonic mucosa: a potential role for these agents in the pathogenesis of ulcerative colitis.

Authors:  S U Christl; H D Eisner; G Dusel; H Kasper; W Scheppach
Journal:  Dig Dis Sci       Date:  1996-12       Impact factor: 3.199

2.  Two's company, three's a crowd: can H2S be the third endogenous gaseous transmitter?

Authors:  Rui Wang
Journal:  FASEB J       Date:  2002-11       Impact factor: 5.191

Review 3.  The emerging roles of hydrogen sulfide in the gastrointestinal tract and liver.

Authors:  Stefano Fiorucci; Eleonora Distrutti; Giuseppe Cirino; John L Wallace
Journal:  Gastroenterology       Date:  2006-03-06       Impact factor: 22.682

4.  The contribution of sulphate reducing bacteria and 5-aminosalicylic acid to faecal sulphide in patients with ulcerative colitis.

Authors:  M C Pitcher; E R Beatty; J H Cummings
Journal:  Gut       Date:  2000-01       Impact factor: 23.059

5.  Culture-independent analysis of desulfovibrios in the human distal colon of healthy, colorectal cancer and polypectomized individuals.

Authors:  Pauline D Scanlan; Fergus Shanahan; Julian R Marchesi
Journal:  FEMS Microbiol Ecol       Date:  2009-05-09       Impact factor: 4.194

Review 6.  Physiological and pathophysiological roles of hydrogen sulfide in the gastrointestinal tract.

Authors:  John L Wallace
Journal:  Antioxid Redox Signal       Date:  2010-05-01       Impact factor: 8.401

7.  Real-time polymerase chain reaction quantification of specific butyrate-producing bacteria, Desulfovibrio and Enterococcus faecalis in the feces of patients with colorectal cancer.

Authors:  Ramadass Balamurugan; Ethendhar Rajendiran; Sarah George; G Vijay Samuel; Balakrishnan S Ramakrishna
Journal:  J Gastroenterol Hepatol       Date:  2008-07-08       Impact factor: 4.029

8.  Identification and quantitation of mucosal and faecal desulfovibrios using real time polymerase chain reaction.

Authors:  A Fite; G T Macfarlane; J H Cummings; M J Hopkins; S C Kong; E Furrie; S Macfarlane
Journal:  Gut       Date:  2004-04       Impact factor: 23.059

9.  Improved extraction of PCR-quality community DNA from digesta and fecal samples.

Authors:  Zhongtang Yu; Mark Morrison
Journal:  Biotechniques       Date:  2004-05       Impact factor: 1.993

10.  Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes.

Authors:  Jo Vandesompele; Katleen De Preter; Filip Pattyn; Bruce Poppe; Nadine Van Roy; Anne De Paepe; Frank Speleman
Journal:  Genome Biol       Date:  2002-06-18       Impact factor: 13.583
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  17 in total

1.  Dietary red meat aggravates dextran sulfate sodium-induced colitis in mice whereas resistant starch attenuates inflammation.

Authors:  Richard K Le Leu; Graeme P Young; Ying Hu; Jean Winter; Michael A Conlon
Journal:  Dig Dis Sci       Date:  2013-08-29       Impact factor: 3.199

Review 2.  Bacterial symbioses of the medicinal leech Hirudo verbana.

Authors:  Michael C Nelson; Joerg Graf
Journal:  Gut Microbes       Date:  2012-05-10

3.  Butyrylated starch intake can prevent red meat-induced O6-methyl-2-deoxyguanosine adducts in human rectal tissue: a randomised clinical trial.

Authors:  Richard K Le Leu; Jean M Winter; Claus T Christophersen; Graeme P Young; Karen J Humphreys; Ying Hu; Silvia W Gratz; Rosalind B Miller; David L Topping; Anthony R Bird; Michael A Conlon
Journal:  Br J Nutr       Date:  2015-06-17       Impact factor: 3.718

4.  Impact of cystic fibrosis disease on archaea and bacteria composition of gut microbiota.

Authors:  Francesco Miragoli; Sara Federici; Susanna Ferrari; Andrea Minuti; Annalisa Rebecchi; Eugenia Bruzzese; Vittoria Buccigrossi; Alfredo Guarino; Maria Luisa Callegari
Journal:  FEMS Microbiol Ecol       Date:  2016-11-02       Impact factor: 4.194

5.  Abundance and Diversity of Hydrogenotrophic Microorganisms in the Infant Gut before the Weaning Period Assessed by Denaturing Gradient Gel Electrophoresis and Quantitative PCR.

Authors:  Valeria Sagheddu; Vania Patrone; Francesco Miragoli; Lorenzo Morelli
Journal:  Front Nutr       Date:  2017-06-26

6.  Differential effects of antiretrovirals on microbial translocation and gut microbiota composition of HIV-infected patients.

Authors:  María J Villanueva-Millán; Patricia Pérez-Matute; Emma Recio-Fernández; José M Lezana Rosales; José A Oteo
Journal:  J Int AIDS Soc       Date:  2017-03-09       Impact factor: 5.396

7.  Characterizing the distribution and rates of microbial sulfate reduction at Middle Valley hydrothermal vents.

Authors:  Kiana L Frank; Daniel R Rogers; Heather C Olins; Charles Vidoudez; Peter R Girguis
Journal:  ISME J       Date:  2013-03-28       Impact factor: 10.302

8.  Xylo-oligosaccharides and inulin affect genotoxicity and bacterial populations differently in a human colonic simulator challenged with soy protein.

Authors:  Claus T Christophersen; Anne Petersen; Tine R Licht; Michael A Conlon
Journal:  Nutrients       Date:  2013-09-23       Impact factor: 5.717

9.  Geomicrobiological linkages between short-chain alkane consumption and sulfate reduction rates in seep sediments.

Authors:  Arpita Bose; Daniel R Rogers; Melissa M Adams; Samantha B Joye; Peter R Girguis
Journal:  Front Microbiol       Date:  2013-12-12       Impact factor: 5.640

10.  Lactate-utilizing community is associated with gut microbiota dysbiosis in colicky infants.

Authors:  Van T Pham; Christophe Lacroix; Christian P Braegger; Christophe Chassard
Journal:  Sci Rep       Date:  2017-09-11       Impact factor: 4.379
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