| Literature DB >> 21460112 |
Min Peng1, Alexandra H Smith, Thomas G Rehberger.
Abstract
A quantitative PCR (qPCR) assay targeting a gene identified by suppressive subtractive hybridization (SSH) was developed to detect Propionibacterium acidipropionici P169, with a threshold of 10(4) CFU/U of dairy feed or rumen fluid. The report is the first using a molecular marker generated by SSH to quantify a bacterial strain in environmental samples.Entities:
Mesh:
Substances:
Year: 2011 PMID: 21460112 PMCID: PMC3127586 DOI: 10.1128/AEM.02586-10
Source DB: PubMed Journal: Appl Environ Microbiol ISSN: 0099-2240 Impact factor: 4.792