AIMS: To investigate the effect of the oxidative stress of ozone on the microbial inactivation, cell membrane integrity and permeability and morphology changes of Escherichia coli. METHODS AND RESULTS: Escherichia coli BW 25113 and its isogenic mutants in soxR, soxS, oxyR, rpoS and dnaK genes were treated with ozone at a concentration of 6 μg ml⁻¹ for a period up to 240 s. A significant effect of ozone exposure on microbial inactivation was observed. After ozonation, minor effects on the cell membrane integrity and permeability were observed, while scanning electron microscopy analysis showed slightly altered cell surface structure. CONCLUSIONS: The results of this study suggest that cell lysis was not the major mechanism of microbial inactivation. The deletion of oxidative stress-related genes resulted in increased susceptibility of E. coli cells to ozone treatment, implying that they play an important role for protection against the radicals produced by ozone. However, DnaK that has previously been shown to protect against oxidative stress did not protect against ozone treatment in this study. Furthermore, RpoS was important for the survival against ozone. SIGNIFICANCE AND IMPACT OF THE STUDY: This study provides important information about the role of oxidative stress in the responses of E. coli during ozonation.
AIMS: To investigate the effect of the oxidative stress of ozone on the microbial inactivation, cell membrane integrity and permeability and morphology changes of Escherichia coli. METHODS AND RESULTS:Escherichia coli BW 25113 and its isogenic mutants in soxR, soxS, oxyR, rpoS and dnaK genes were treated with ozone at a concentration of 6 μg ml⁻¹ for a period up to 240 s. A significant effect of ozone exposure on microbial inactivation was observed. After ozonation, minor effects on the cell membrane integrity and permeability were observed, while scanning electron microscopy analysis showed slightly altered cell surface structure. CONCLUSIONS: The results of this study suggest that cell lysis was not the major mechanism of microbial inactivation. The deletion of oxidative stress-related genes resulted in increased susceptibility of E. coli cells to ozone treatment, implying that they play an important role for protection against the radicals produced by ozone. However, DnaK that has previously been shown to protect against oxidative stress did not protect against ozone treatment in this study. Furthermore, RpoS was important for the survival against ozone. SIGNIFICANCE AND IMPACT OF THE STUDY: This study provides important information about the role of oxidative stress in the responses of E. coli during ozonation.
Authors: Kaća Piletić; Bruno Kovač; Marko Perčić; Jure Žigon; Dalibor Broznić; Ljerka Karleuša; Sanja Lučić Blagojević; Martina Oder; Ivana Gobin Journal: Int J Environ Res Public Health Date: 2022-05-19 Impact factor: 4.614
Authors: Batbileg Bor; Nicole Poweleit; Justin S Bois; Lujia Cen; Joseph K Bedree; Z Hong Zhou; Robert P Gunsalus; Renate Lux; Jeffrey S McLean; Xuesong He; Wenyuan Shi Journal: Microb Ecol Date: 2015-11-23 Impact factor: 4.552
Authors: Karyne Rangel; Fellipe O Cabral; Guilherme C Lechuga; João P R S Carvalho; Maria H S Villas-Bôas; Victor Midlej; Salvatore G De-Simone Journal: Molecules Date: 2022-06-22 Impact factor: 4.927
Authors: Junjie Li; Christoph Rumancev; Holger V Lutze; Torsten C Schmidt; Axel Rosenhahn; Oliver J Schmitz Journal: Anal Bioanal Chem Date: 2020-07-16 Impact factor: 4.142
Authors: Karyne Rangel; Fellipe O Cabral; Guilherme C Lechuga; João P R S Carvalho; Maria H S Villas-Bôas; Victor Midlej; Salvatore G De-Simone Journal: Microorganisms Date: 2021-12-26