Literature DB >> 21455933

Altered substrate specificity of the gluco-oligosaccharide oxidase from Acremonium strictum.

Maryam Foumani1, Thu V Vuong, Emma R Master.   

Abstract

A gluco-oligosaccharide oxidase (GOOX) from Acremonium strictum type strain CBS 346.70 was cloned and expressed in Pichia pastoris. The recombinant protein, GOOX-VN, contained fifteen amino acid substitutions compared with the previously reported A. strictum GOOX. These two enzymes share 97% sequence identity; however, only GOOX-VN oxidized xylose, galactose, and N-acetylglucosamine. Besides monosaccharides, GOOX-VN oxidized xylo-oligosaccharides, including xylobiose and xylotriose with similar catalytic efficiency as for cello-oligosaccharides. Of three mutant enzymes that were created in GOOX-VN to improve substrate specificity, Y300A and Y300N doubled kcat values for monosaccharide and oligosaccharide substrates. With this novel substrate specificity, GOOX-VN and its variants are particularly valuable for oxidative modification of cello- and xylo-oligosaccharides.
Copyright © 2011 Wiley Periodicals, Inc.

Entities:  

Keywords:  Acremonium strictum; carbohydrate docking; gluco-oligosaccharide oxidase; protein engineering; substrate specificity

Mesh：

Substances：

Year:  2011        PMID： 21455933     DOI： 10.1002/bit.23149

Source DB:  PubMed          Journal:  Biotechnol Bioeng        ISSN： 0006-3592            Impact factor:   4.530


  7 in total

1.  A Novel Colletotrichum graminicola Raffinose Oxidase in the AA5 Family.

Authors:  Martina Andberg; Filip Mollerup; Kirsti Parikka; Sanna Koutaniemi; Harry Boer; Minna Juvonen; Emma Master; Maija Tenkanen; Kristiina Kruus
Journal:  Appl Environ Microbiol       Date:  2017-09-29       Impact factor: 4.792

2.  Enhanced Polysaccharide Binding and Activity on Linear β-Glucans through Addition of Carbohydrate-Binding Modules to Either Terminus of a Glucooligosaccharide Oxidase.

Authors:  Maryam Foumani; Thu V Vuong; Benjamin MacCormick; Emma R Master
Journal:  PLoS One       Date:  2015-05-01       Impact factor: 3.240

3.  Xylo- and cello-oligosaccharide oxidation by gluco-oligosaccharide oxidase from Sarocladium strictum and variants with reduced substrate inhibition.

Authors:  Thu V Vuong; Arja-Helena Vesterinen; Maryam Foumani; Minna Juvonen; Jukka Seppälä; Maija Tenkanen; Emma R Master
Journal:  Biotechnol Biofuels       Date:  2013-10-12       Impact factor: 6.040

4.  Direct comparison of gluco-oligosaccharide oxidase variants and glucose oxidase: substrate range and H2O2 stability.

Authors:  Thu V Vuong; Maryam Foumani; Benjamin MacCormick; Rachel Kwan; Emma R Master
Journal:  Sci Rep       Date:  2016-11-21       Impact factor: 4.379

5.  Insights into the genome and secretome of Fusarium metavorans DSM105788 by cultivation on agro-residual biomass and synthetic nutrient sources.

Authors:  Sophie C Brandt; Hévila Brognaro; Arslan Ali; Bernhard Ellinger; Katharina Maibach; Martin Rühl; Carsten Wrenger; Hartmut Schlüter; Wilhelm Schäfer; Christian Betzel; Stefan Janssen; Martin Gand
Journal:  Biotechnol Biofuels       Date:  2021-03-20       Impact factor: 6.040

6.  Fusion of a xylan-binding module to gluco-oligosaccharide oxidase increases activity and promotes stable immobilization.

Authors:  Thu V Vuong; Emma R Master
Journal:  PLoS One       Date:  2014-04-15       Impact factor: 3.240

7.  Enzymatic production of 4-O-methyl d-glucaric acid from hardwood xylan.

Authors:  Thu V Vuong; Emma R Master
Journal:  Biotechnol Biofuels       Date:  2020-03-13       Impact factor: 6.040
  7 in total

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