Contributions of microstructure and chemical composition to the mechanical properties of dentin.

The influence of microstructural variations and chemical composition to the mechanical properties and apparent flaw sensitivity of dentin were evaluated. Rectangular beams (N = 80) of the deep and superficial coronal dentin were prepared from virgin 3rd molars; twenty beams of each region were nominally flaw free and the remainder possessed a single "surface flaw" via a Vickers indentation. Mechanical properties were estimated in four-point flexure and examined using Weibull statistics. Fourier Transform Infrared Microspectroscopy in Reflectance Mode (FTIR-RM) was used to quantify the relative mineral to collagen ratios. Results showed that the average flexural strength, and strain and energy to fracture of the deep dentin beams were significantly lower (P < 0.005) than for the superficial dentin. While the deep dentin exhibited the highest mineral/collagen ratio and lowest damage tolerance, there was no significant effect of the surface flaws. Weibull analyses suggest that deep dentin possesses a larger distribution of intrinsic flaw sizes that contributes to the location dependence in strength.