SirSupporting the work of Jain et al. (1:172-174, 2010), we add the following.Authors referred the first report of gold in semen of 1981, which was carried out by employing neutron activation analysis.[1]Processing semen sample for atomic absorption spectrophotometric study was modified and has been in regular use.[2]By employing atomic absorption spectrophotometer, we estimated gold in normal and pathological semen samples, resulting in a clue that gold is essential for normal functioning of spermatozoa.[34]By using Energy Dispersive X-ray Analysis (EDAX) on electron microscope, we observed gold inside and outside human spermatozoa.[5]The presence of the metal in human male system was studied with X-ray diffraction analysis method. Gold was present throughout human male genital system except in seminal vesicles.[6] Initially, by employing mass emission spectroscope, we had shown that gold reached semen from caput epididymis.[7] The gold remained in a trimetal complex with copper and zinc.[6] Direct couple plasma emission spectroscope failed to show gold in semen.[8]“Suvarna bhasma” (Gold Ash) was found to be very useful in the treatment of different pathological conditions.[9]Gold may be entering body through drinking water and food. Study on this line was designed. It was present in human semen of both sets of people: i) where soil had gold content and ii) where soil had no measurable amount of gold. Gold content in semen was more in the people where gold was present in soil.[10] Further, the difference in the quality of semen surrounding a gold mine and away will be published elsewhere.