| Literature DB >> 21453676 |
Agnieszka Zdybicka-Barabas1, Barbara Januszanis, Pawel Mak, Małgorzata Cytryńska.
Abstract
Apolipophorin III (apoLp-III) is an abundant hemolymph protein involved in lipid transport and immune response in insects. As revealed by LIVE/DEAD staining, incubation of Gram-negative and Gram-positive bacteria in the presence of Galleria mellonella apoLp-III led to growth inhibition of selected bacteria. An atomic force microscopy (AFM) study of bacterial cells after apoLp-III treatment showed considerable alterations in the cell surface of Bacillus circulans, Klebsiella pneumoniae and Salmonella typhimurium. Our results clearly demonstrate that apoLp-III disturbed the proper structure of the bacterial cell surface. The alterations were dissimilar to those caused by cationic antimicrobial peptide, cecropin B, suggesting a different mode of action against bacteria. The present results indicate that AFM provides a powerful tool for studying the interactions of apoLp-III with microbial cells.Entities:
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Year: 2011 PMID： 21453676 DOI： 10.1016/j.bbamem.2011.03.013
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002