Feng Wang1, Yue Zhu. 1. Department of Orthopaedics, The First Hospital of China Medical University, No. 155 Nanjingbei Road, Heping District, Shenyang 110001, People's Republic of China.
Abstract
BACKGROUND: During the process of degenerative aging of the intervertebral disc (IVD), the extracellular matrix (ECM) environment changes, with osmolarity and oxygen (O(2)) concentration important components of such changes. The IVD cells respond to maintain the homeostasis and function of the IVD by several mechanisms. Aquaporin-1 (AQP-1) is a transmembrane channel protein that is permeable to water and O(2), which prevents rapid volume deformation under osmotic stress and facilitates O(2) diffusion across the plasma membrane. One hypothesis is that AQP-1 has potential roles in aging degeneration of IVDs. METHODS: In this study, AQP-1 expression levels were investigated in aging rabbit nucleus pulposus (NP) cells using immunohistochemistry and Western blotting in vivo, and different osmolarities and O(2) concentrations in vitro by quantitative real-time PCR. RESULTS: The results showed that AQP-1 was expressed at different levels in aging rabbit's NPs and AQP-1 was regulated by the NP cells in different ECM environmental conditions. AQP-1 was downregulated under hypo-osmotic stress to prevent rapid swelling deformation and was upregulated under hypoxic stress to facilitate O(2) utilization. CONCLUSION: It is suggested that AQP-1 may reflect the status of aged IVDs and have a potential role in reflecting the adaptability of NP cells under different adverse ECM environments in aging degenerated IVDs.
BACKGROUND: During the process of degenerative aging of the intervertebral disc (IVD), the extracellular matrix (ECM) environment changes, with osmolarity and oxygen (O(2)) concentration important components of such changes. The IVD cells respond to maintain the homeostasis and function of the IVD by several mechanisms. Aquaporin-1 (AQP-1) is a transmembrane channel protein that is permeable to water and O(2), which prevents rapid volume deformation under osmotic stress and facilitates O(2) diffusion across the plasma membrane. One hypothesis is that AQP-1 has potential roles in aging degeneration of IVDs. METHODS: In this study, AQP-1 expression levels were investigated in aging rabbit nucleus pulposus (NP) cells using immunohistochemistry and Western blotting in vivo, and different osmolarities and O(2) concentrations in vitro by quantitative real-time PCR. RESULTS: The results showed that AQP-1 was expressed at different levels in aging rabbit's NPs and AQP-1 was regulated by the NP cells in different ECM environmental conditions. AQP-1 was downregulated under hypo-osmotic stress to prevent rapid swelling deformation and was upregulated under hypoxic stress to facilitate O(2) utilization. CONCLUSION: It is suggested that AQP-1 may reflect the status of aged IVDs and have a potential role in reflecting the adaptability of NP cells under different adverse ECM environments in aging degenerated IVDs.
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