BACKGROUND: A reciprocal link between inflammation, oxidative/nitrosative stress, and endothelial dysfunction has been postulated in chronic heart failure (CHF). The endothelial repair mechanisms involved remain to be determined. Our aim was to investigate whether there are detectable signs of ongoing angiogenesis in serum of CHF patients and to evaluate the correlation with indexes of haemodynamic and functional impairment. METHODS AND RESULTS: Enzyme-linked immunosorbent assay tests were used to quantify angiogenin, angiopoietin-1, angiopoietin-2, vascular endothelial growth factor, Tie-2, and brain natriuretic peptide in serum of 87 patients with CHF of increasing severity according to New York Heart Association (NYHA; class I, n = 8; II, n = 45; and III, n = 34) and in 14 healthy subjects matched for age and sex. Angiogenin, angiopoietin-2, and Tie-2 were significantly increased in CHF of increasing severity (Kruskal-Wallis: p = 0.0004, p < 0.0001, and p = 0.017, respectively). Angiopoietin-2 was inversely correlated with the 6-min walking test (r = -0.65, p < 0.0001), peak oxygen consumption (VO(2max); r = -0.57, p = 0.0002), and deceleration time (r = -0.61, p < 0.0001). Multiple regression analysis showed that angiopoietin-2 was mainly associated with VO(2max) (p = 0.018). The angiopoietin-2 area under the receiver operating characteristic curve for CHF diagnosis was 0.94 (95% CI 0.88-0.99; p < 0.001). CONCLUSIONS: These data demonstrate that angiopoietin-2 and selected serum markers of angiogenesis progressively increase with haemodynamic and functional decline in CHF.
BACKGROUND: A reciprocal link between inflammation, oxidative/nitrosative stress, and endothelial dysfunction has been postulated in chronic heart failure (CHF). The endothelial repair mechanisms involved remain to be determined. Our aim was to investigate whether there are detectable signs of ongoing angiogenesis in serum of CHFpatients and to evaluate the correlation with indexes of haemodynamic and functional impairment. METHODS AND RESULTS: Enzyme-linked immunosorbent assay tests were used to quantify angiogenin, angiopoietin-1, angiopoietin-2, vascular endothelial growth factor, Tie-2, and brain natriuretic peptide in serum of 87 patients with CHF of increasing severity according to New York Heart Association (NYHA; class I, n = 8; II, n = 45; and III, n = 34) and in 14 healthy subjects matched for age and sex. Angiogenin, angiopoietin-2, and Tie-2 were significantly increased in CHF of increasing severity (Kruskal-Wallis: p = 0.0004, p < 0.0001, and p = 0.017, respectively). Angiopoietin-2 was inversely correlated with the 6-min walking test (r = -0.65, p < 0.0001), peak oxygen consumption (VO(2max); r = -0.57, p = 0.0002), and deceleration time (r = -0.61, p < 0.0001). Multiple regression analysis showed that angiopoietin-2 was mainly associated with VO(2max) (p = 0.018). The angiopoietin-2 area under the receiver operating characteristic curve for CHF diagnosis was 0.94 (95% CI 0.88-0.99; p < 0.001). CONCLUSIONS: These data demonstrate that angiopoietin-2 and selected serum markers of angiogenesis progressively increase with haemodynamic and functional decline in CHF.
Authors: Manuel J Richter; Ralph Schermuly; Werner Seeger; Youlan Rao; Hossein A Ghofrani; Henning Gall Journal: Pulm Circ Date: 2016-12 Impact factor: 3.017
Authors: Janine Pöss; Christian Ukena; Ingrid Kindermann; Peter Ehrlich; Georg Fuernau; Sebastian Ewen; Felix Mahfoud; Steffen Kriechbaum; Michael Böhm; Andreas Link Journal: Clin Res Cardiol Date: 2014-11-19 Impact factor: 5.460
Authors: Quinn S Wells; Deepak K Gupta; J Gustav Smith; Sean P Collins; Alan B Storrow; Jane Ferguson; Maya Landenhed Smith; Jill M Pulley; Sarah Collier; Xiaoming Wang; Dan M Roden; Robert E Gerszten; Thomas J Wang Journal: J Am Coll Cardiol Date: 2019-05-07 Impact factor: 24.094
Authors: Kazuyo Yamaji-Kegan; Eiki Takimoto; Ailan Zhang; Noah C Weiner; Lucas W Meuchel; Alan E Berger; Chris Cheadle; Roger A Johns Journal: Am J Physiol Lung Cell Mol Physiol Date: 2014-05-02 Impact factor: 5.464
Authors: Alexander Lukasz; Gernot Beutel; Philipp Kümpers; Agnieszka Denecke; Mechthild Westhoff-Bleck; Bernhard Schieffer; Johann Bauersachs; Jan T Kielstein; Oktay Tutarel Journal: PLoS One Date: 2013-06-24 Impact factor: 3.240
Authors: Brandon S Peplinski; Brian A Houston; David A Bluemke; Steven M Kawut; Todd M Kolb; Richard A Kronmal; Joao A C Lima; David D Ralph; Samuel G Rayner; Zachary L Steinberg; Ryan J Tedford; Peter J Leary Journal: J Card Fail Date: 2021-04-17 Impact factor: 6.592
Authors: Courtney Premer; Arnon Blum; Michael A Bellio; Ivonne Hernandez Schulman; Barry E Hurwitz; Meela Parker; Christopher R Dermarkarian; Darcy L DiFede; Wayne Balkan; Aisha Khan; Joshua M Hare Journal: EBioMedicine Date: 2015-03-28 Impact factor: 8.143