PURPOSE: To evaluate the apparent diffusion coefficient (ADC) values of liver in a murine model of non-alcoholic steatohepatitis using 11.7 Tesla (T) MRI. MATERIALS AND METHODS: This animal study was IACUC approved. Seventeen male C57BL/6 mice were divided into control (n = 3) and experimental groups (n = 14) fed a methionine-deficient choline-deficient (MCD) diet to induce steatohepatitis. Livers underwent ex vivo diffusion-weighted MR imaging and ADC maps were calculated. A pathologist determined subjective scores of steatosis, classified from 0 to 3. Digital image analysis was used to determine percentage areas of steatosis. Graphs comparing ADC to subjective and digital image analysis (DIA) determinations of steatosis were plotted. RESULTS: Subjective assessments of steatosis ranged up to values of 3 and DIA determined areas of steatosis to range up to approximately 16%. ADC values approximated 800 × 10(-6) mm(2) /s (range, 749-811 × 10(-6) mm(2) /s, mean 786 × 10(-6) mm(2) /s) in controls and 500 × 10(-6) mm(2) /s (range, 478-733 × 10(-6) mm(2) /s, mean 625 × 10(-6) mm(2) /s) in experimental mice. Moderate correlation between ADC and subjective scores of steatosis (R = -0.56) was observed. Strong correlation between ADC values and percentage areas of steatosis was between ADC values and percentage areas of steatosis was observed greater (R = -0.81) and very strong correlation was observed with the exclusion of a single outlying data point (R = -0.91). CONCLUSION: Based on the comparison of ADC values and steatosis determinations by DIA, increasing degrees of steatosis are seen to result in decreased hepatic ADC values.
PURPOSE: To evaluate the apparent diffusion coefficient (ADC) values of liver in a murine model of non-alcoholic steatohepatitis using 11.7 Tesla (T) MRI. MATERIALS AND METHODS: This animal study was IACUC approved. Seventeen male C57BL/6 mice were divided into control (n = 3) and experimental groups (n = 14) fed a methionine-deficient choline-deficient (MCD) diet to induce steatohepatitis. Livers underwent ex vivo diffusion-weighted MR imaging and ADC maps were calculated. A pathologist determined subjective scores of steatosis, classified from 0 to 3. Digital image analysis was used to determine percentage areas of steatosis. Graphs comparing ADC to subjective and digital image analysis (DIA) determinations of steatosis were plotted. RESULTS: Subjective assessments of steatosis ranged up to values of 3 and DIA determined areas of steatosis to range up to approximately 16%. ADC values approximated 800 × 10(-6) mm(2) /s (range, 749-811 × 10(-6) mm(2) /s, mean 786 × 10(-6) mm(2) /s) in controls and 500 × 10(-6) mm(2) /s (range, 478-733 × 10(-6) mm(2) /s, mean 625 × 10(-6) mm(2) /s) in experimental mice. Moderate correlation between ADC and subjective scores of steatosis (R = -0.56) was observed. Strong correlation between ADC values and percentage areas of steatosis was between ADC values and percentage areas of steatosis was observed greater (R = -0.81) and very strong correlation was observed with the exclusion of a single outlying data point (R = -0.91). CONCLUSION: Based on the comparison of ADC values and steatosis determinations by DIA, increasing degrees of steatosis are seen to result in decreased hepatic ADC values.
Authors: Paul Murphy; Jonathan Hooker; Brandon Ang; Tanya Wolfson; Anthony Gamst; Mark Bydder; Michael Middleton; Michael Peterson; Cynthia Behling; Rohit Loomba; Claude Sirlin Journal: J Magn Reson Imaging Date: 2014-09-25 Impact factor: 4.813
Authors: Helena S Leitão; Sabrina Doblas; Gaspard d'Assignies; Philippe Garteiser; Jean-Luc Daire; Valérie Paradis; Carlos F G C Geraldes; Valérie Vilgrain; Bernard E Van Beers Journal: Eur Radiol Date: 2012-09-01 Impact factor: 5.315
Authors: Paul Manning; Paul Murphy; Kang Wang; Jonathan Hooker; Tanya Wolfson; Michael S Middleton; Kimberly P Newton; Cynthia Behling; Hannah I Awai; Janis Durelle; Melissa N Paiz; Jorge E Angeles; Diana De La Pena; J Allen McCutchan; Jeffrey B Schwimmer; Claude B Sirlin Journal: J Magn Reson Imaging Date: 2017-02-22 Impact factor: 4.813
Authors: Irene Marco-Rius; Jeremy W Gordon; Aras N Mattis; Robert Bok; Romelyn Delos Santos; Subramanian Sukumar; Peder E Z Larson; Daniel B Vigneron; Michael A Ohliger Journal: J Magn Reson Imaging Date: 2017-04-17 Impact factor: 4.813
Authors: Ilias I Giannakopoulos; Georgy D Guryev; José E C Serrallés; Ioannis P Georgakis; Luca Daniel; Jacob K White; Riccardo Lattanzi Journal: IEEE Trans Antennas Propag Date: 2021-06-25 Impact factor: 4.388