| Literature DB >> 21448704 |
Fengju Zhang1, Xia Wu, Jinhua Zhan.
Abstract
A sensitive and selective method for the trace determination of 3, 3', 4, 4'-tetrachlorobiphenyl (PCB77) by using bovine serum albumin (BSA) as a fluorescence probe was introduced. Under optimum conditions, the enhanced fluorescence intensity was proportional to the concentration of polychlorinated biphenyls in the range of 8.9 × 10(-8)-5.0 × 10(-6) mol L(-1) for PCB77, and 5.0 × 10(-7)-5.0 × 10(-6) mol L(-1) for 2, 2', 5, 5'-tetrachlorbiphenyl (PCB52). The detection limits (S/N = 3) of PCB77 and PCB52 were 2.6 × 10(-8) mol L(-1) and 2.9 × 10(-7) mol L(-1), respectively. Furthermore, the fluorescence enhancement mechanism was discussed in detail. Results indicated that fluorescence enhancement of the system originated from the formation of BSA-PCBs complexes. In addition, PCBs were mainly bound to the tyrosine residues in BSA molecules.Entities:
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Year: 2011 PMID： 21448704 DOI： 10.1007/s10895-011-0880-9
Source DB: PubMed Journal: J Fluoresc ISSN： 1053-0509 Impact factor: 2.217